Development of selection marker-free transgenic potato plants with enhanced tolerance to oxidative stress View Full Text


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Article Info

DATE

2008-11

AUTHORS

Raza Ahmad, Yun-Hee Kim, Myoung Duck Kim, Minh-Ngoc Phung, Won-Il Chung, Haeng-Soon Lee, Sang-Soo Kwak, Suk-Yoon Kwon

ABSTRACT

A binary vector devoid of a plant selection-marker gene (designated as pSSA-F) was constructed to overcome bio-safety concerns about genetically modified plants. This vector carried chloroplast-targeted superoxide dismutase (SOD) and ascorbate peroxidase (APX) genes under the control of an oxidative stress-inducible(SWPA2) promoter, and was utilized to transform potato (Solanum tuberosum L.). Integration of these foreign genes into transgenic plants was primarily performed via PCR with genomic DNA. Twelve marker-free transgenic lines were obtained by inoculating stem explants. The maximum transformation efficiency was 6.25% and averaged 2.2%. Successful integration of the SOD and APX genes rendered transgenic plants tolerant to methyl viologen-mediated oxidative stress at the leaf-disc and whole-plant levels. Our findings suggest that this technique for developing selection marker-free transgenic plants is feasible and can be employed with other crop species. More... »

PAGES

401-407

References to SciGraph publications

  • 2003-11. A framework for designing transgenic crops—science, safety and citizen's concerns in NATURE BIOTECHNOLOGY
  • 2001-02. Chemical-regulated, site-specific DNA excision in transgenic plants in NATURE BIOTECHNOLOGY
  • 2001-07. Systems for the removal of a selection marker and their combination with a positive marker in PLANT CELL REPORTS
  • 1999-03. Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor in NATURE BIOTECHNOLOGY
  • 2003-04. A novel oxidative stress-inducible peroxidase promoter from sweetpotato: molecular cloning and characterization in transgenic tobacco plants and cultured cells in PLANT MOLECULAR BIOLOGY
  • 2004-11. Co-transformation using a negative selectable marker gene for the production of selectable marker gene-free transgenic plants in THEORETICAL AND APPLIED GENETICS
  • 2005-12. Rapid and Reliable Method of Extracting DNA and RNA from Sweetpotato, Ipomoea batatas (L). Lam in BIOTECHNOLOGY LETTERS
  • 1991-05. Agrobacterium-mediated transformation of Solanum tuberosum L. cv. ‘Russet Burbank’ in PLANT CELL REPORTS
  • 1999-05. Selectable marker-free transgenic plants without sexual crossing: transient expression of cre recombinase and use of a conditional lethal dominant gene in PLANT MOLECULAR BIOLOGY
  • 2008-04. Stress-induced expression of choline oxidase in potato plant chloroplasts confers enhanced tolerance to oxidative, salt, and drought stresses in PLANT CELL REPORTS
  • 2003-04. A transformation method for obtaining marker-free plants of a cross-pollinating and vegetatively propagated crop in NATURE BIOTECHNOLOGY
  • 2007-10. A Cre/loxP-mediated self-activating gene excision system to produce marker gene free transgenic soybean plants in PLANT MOLECULAR BIOLOGY
  • 2007-08. Growth and tuberization of transgenic potato plants expressing sense and antisense sequences of Cu/Zn superoxide dismutase from lily chloroplasts in JOURNAL OF PLANT BIOLOGY
  • 1985-10. Growth analyses of eleven potato cultivars grown in the greenhouse under long photoperiods with and without heat stress in AMERICAN JOURNAL OF POTATO RESEARCH
  • 2007-06. Biosafety and risk assessment framework for selectable marker genes in transgenic crop plants: a case of the science not supporting the politics in TRANSGENIC RESEARCH
  • 2008-08. Enhanced tolerance of transgenic potato plants overexpressing nucleoside diphosphate kinase 2 against multiple environmental stresses in TRANSGENIC RESEARCH
  • 1999-07. A mini binary vector series for plant transformation in PLANT MOLECULAR BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf03036060

    DOI

    http://dx.doi.org/10.1007/bf03036060

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1014363049


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