Study of propidium iodide binding to DNA in intact cells by flow cytometry View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-12

AUTHORS

A. Bertuzzi, I. D’Agnano, A. Gandolfi, A. Graziano, G. Starace, P. Ubezio

ABSTRACT

We studied thein situ binding of propidium iodide to DNA in fixed human lymphocytes, using flow cytometry. Experimental data of fluorescence emission vs dye concentration and vs cell concentration were obtained. Data were interpreted by means of two different mathematical models specific for the staining reaction, and the binding parameters were obtained by “best-fitting” of the data. A model based on two classes of binding sites with different affinity constants gave the most satisfactory fitting. The accessibility of thein situ chromatin turned out to be reduced with respect to the nonin situ accessibility for ethidium bromide as reported in the literature. The present study shows the usefulness of the flow-cytometric technique for probing DNA structure in intact cells. More... »

PAGES

257-267

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02990721

DOI

http://dx.doi.org/10.1007/bf02990721

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034999398

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1714351


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