Cloning and characterization of directly amplified antiviral gene interferon alpha-2b (hulfnα-2b) from human leukocytes chromosomal DNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-07

AUTHORS

Javad Behravan, Hassan Ahmadpour

ABSTRACT

Interferons are cytokines that confer resistance to viral infection and inhibit cellular proliferation. The interferon alpha gene from human blood samples was amplified, cloned and expressed inE. coli (BL21). Leukocyte chromosomal DNA was used as a source of template DNA. Using specific primers, the gene for HulFNα-2b was amplified and inserted into theE. coli vector, pET21b, by ligation of theHindIII andBamHI linkers of the vector and insert. The insert was further analyzed by PCR, DNA restriction mapping and sequencing, and expressed in a suitableE. coli strain. The production of this important cellular protein in the laboratory has significant applications in production of the recombinant pharmaceutical proteins. More... »

PAGES

776

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02980148

DOI

http://dx.doi.org/10.1007/bf02980148

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1051250423

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15357007


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