Studies on nutrient requirements and cost-effective supplements for ethanol production by recombinantE. coli View Full Text


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Article Info

DATE

1996-03

AUTHORS

Hugh G. Lawford, Joyce D. Rousseau

ABSTRACT

This article describes a systematic study of the nutritional requirements of a patented recombinant ethanologenic Escherichia coli (11303:pLO1297) and provides cost-effective formulations that are compatible with the production of fuel ethanol in fermentations of lignocellulosic prehydrolysate characterized by high xylose conversion efficiency. A complex and nutrient-rich laboratory medium, Luria broth (LB), provided the benchmark with respect to fermentation performance standard. Xylose fermentation performance was assessed in terms of the target values for operational process parameters established by the US National Renewable Energy Laboratory (NREL)-final ethanol concentration (25 g/L), xylose-to-ethanol conversion efficiency (90%), and volumetric productivity (0.52 g/L.h). Biomass prehydrolysates that are rich in xylose also contain acetic acid, and in anticipation of a need to reduce acetic acid toxicity, the fermentors were operated with a pH control set-point of 7.0 Growth and fermentation in the minimal defined salts (DS) medium was only about 15% compared to the reference medium. Amendment of the minimal medium containing 6 wt% xylose with both vitamins and amino acids resulted in improved growth, but the volume productivity (0.59 g/L.h) was still only about 54% of that with LB (1.1g/L.h). Formulations directed at cost reduction through the use of less expensive commercial complex nutritional supplements were within 90% of the NREL process target with respect to yield and provided a productivity at about 80% of the LB medium, but were not economical. Corn steep liquor (CSL) at about 7-8 g/L was shown to be a complete source of nutritional requirements and supported a fermentation performance approaching that of LB. At a cost of CSL of $50/t(dry wt), the economic impact of using this amount CSL as the sole nutritional supplement in a cellulosic ethanol plant was estimated to be about 4 cents/gal of ethanol. More... »

PAGES

307

References to SciGraph publications

  • 1993-09. The effect of acetic acid on fuel ethanol production byZymomonas in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1995-09. Comparative energetics of glucose and xylose metabolism in ethanologenic recombinantEscherichia coli B in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1992-09. Conversion of hydrolysates of corn cobs and hulls into ethanol by recombinantEscherichia coli B containing integrated genes for ethanol production in BIOTECHNOLOGY LETTERS
  • 1996-03. Factors contributing to the loss of ethanologenicity ofEscherichia coli B recombinants pL0I297 and KO11 in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1995-09. Fermentation of sugars in organe peel hydrolysates to ethanol by recombinantEscherichia coli KO11 in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1990-03. Ethanol in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1991-03. Fuel ethanol from hardwood hemicellulose hydrolysate by genetically engineeredEscherichia coli B carrying genes fromZymomonas mobilis in BIOTECHNOLOGY LETTERS
  • 1989-01. Xylose fermentation in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1989. Production of ethanol from lignocellulosic materials using thermophilic bacteria: Critical evaluation of potential and review in LIGNOCELLULOSIC MATERIALS
  • 1991-03. Ethanol production by recombinantescherichia coli carrying genes from zymomonas mobilis in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1994-03. Fermentation of galacturonic acid and other sugars in orange peel hydrolysates by the ethanologenic strain of Escherichia coli in BIOTECHNOLOGY LETTERS
  • 1993-09. High cell density cultivation of Escherichia coli W using sucrose as a carbon source in BIOTECHNOLOGY LETTERS
  • 1993-05. A comparative study of glucose conversion to ethanol by Zymomonas mobilis and a recombinant Escherichia coli in BIOTECHNOLOGY LETTERS
  • 1994-02. Comparison of corn steep liquor with other nutrients in the fermentation of D-Xylose by Pichia stipitis CBS 6054 in BIOTECHNOLOGY LETTERS
  • 1992-03. Effect of acetic acid on xylose conversion to ethanol by genetically engineeredE. coli in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • Identifiers

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    42 schema:description This article describes a systematic study of the nutritional requirements of a patented recombinant ethanologenic Escherichia coli (11303:pLO1297) and provides cost-effective formulations that are compatible with the production of fuel ethanol in fermentations of lignocellulosic prehydrolysate characterized by high xylose conversion efficiency. A complex and nutrient-rich laboratory medium, Luria broth (LB), provided the benchmark with respect to fermentation performance standard. Xylose fermentation performance was assessed in terms of the target values for operational process parameters established by the US National Renewable Energy Laboratory (NREL)-final ethanol concentration (25 g/L), xylose-to-ethanol conversion efficiency (90%), and volumetric productivity (0.52 g/L.h). Biomass prehydrolysates that are rich in xylose also contain acetic acid, and in anticipation of a need to reduce acetic acid toxicity, the fermentors were operated with a pH control set-point of 7.0 Growth and fermentation in the minimal defined salts (DS) medium was only about 15% compared to the reference medium. Amendment of the minimal medium containing 6 wt% xylose with both vitamins and amino acids resulted in improved growth, but the volume productivity (0.59 g/L.h) was still only about 54% of that with LB (1.1g/L.h). Formulations directed at cost reduction through the use of less expensive commercial complex nutritional supplements were within 90% of the NREL process target with respect to yield and provided a productivity at about 80% of the LB medium, but were not economical. Corn steep liquor (CSL) at about 7-8 g/L was shown to be a complete source of nutritional requirements and supported a fermentation performance approaching that of LB. At a cost of CSL of $50/t(dry wt), the economic impact of using this amount CSL as the sole nutritional supplement in a cellulosic ethanol plant was estimated to be about 4 cents/gal of ethanol.
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