Studies on nutrient requirements and cost-effective supplements for ethanol production by recombinantE. coli View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-03

AUTHORS

Hugh G. Lawford, Joyce D. Rousseau

ABSTRACT

This article describes a systematic study of the nutritional requirements of a patented recombinant ethanologenic Escherichia coli (11303:pLO1297) and provides cost-effective formulations that are compatible with the production of fuel ethanol in fermentations of lignocellulosic prehydrolysate characterized by high xylose conversion efficiency. A complex and nutrient-rich laboratory medium, Luria broth (LB), provided the benchmark with respect to fermentation performance standard. Xylose fermentation performance was assessed in terms of the target values for operational process parameters established by the US National Renewable Energy Laboratory (NREL)-final ethanol concentration (25 g/L), xylose-to-ethanol conversion efficiency (90%), and volumetric productivity (0.52 g/L.h). Biomass prehydrolysates that are rich in xylose also contain acetic acid, and in anticipation of a need to reduce acetic acid toxicity, the fermentors were operated with a pH control set-point of 7.0 Growth and fermentation in the minimal defined salts (DS) medium was only about 15% compared to the reference medium. Amendment of the minimal medium containing 6 wt% xylose with both vitamins and amino acids resulted in improved growth, but the volume productivity (0.59 g/L.h) was still only about 54% of that with LB (1.1g/L.h). Formulations directed at cost reduction through the use of less expensive commercial complex nutritional supplements were within 90% of the NREL process target with respect to yield and provided a productivity at about 80% of the LB medium, but were not economical. Corn steep liquor (CSL) at about 7-8 g/L was shown to be a complete source of nutritional requirements and supported a fermentation performance approaching that of LB. At a cost of CSL of $50/t(dry wt), the economic impact of using this amount CSL as the sole nutritional supplement in a cellulosic ethanol plant was estimated to be about 4 cents/gal of ethanol. More... »

PAGES

307

References to SciGraph publications

  • 1993-09. The effect of acetic acid on fuel ethanol production byZymomonas in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1995-09. Comparative energetics of glucose and xylose metabolism in ethanologenic recombinantEscherichia coli B in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1992-09. Conversion of hydrolysates of corn cobs and hulls into ethanol by recombinantEscherichia coli B containing integrated genes for ethanol production in BIOTECHNOLOGY LETTERS
  • 1996-03. Factors contributing to the loss of ethanologenicity ofEscherichia coli B recombinants pL0I297 and KO11 in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1995-09. Fermentation of sugars in organe peel hydrolysates to ethanol by recombinantEscherichia coli KO11 in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1990-03. Ethanol in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1991-03. Fuel ethanol from hardwood hemicellulose hydrolysate by genetically engineeredEscherichia coli B carrying genes fromZymomonas mobilis in BIOTECHNOLOGY LETTERS
  • 1989-01. Xylose fermentation in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1989. Production of ethanol from lignocellulosic materials using thermophilic bacteria: Critical evaluation of potential and review in LIGNOCELLULOSIC MATERIALS
  • 1991-03. Ethanol production by recombinantescherichia coli carrying genes from zymomonas mobilis in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1994-03. Fermentation of galacturonic acid and other sugars in orange peel hydrolysates by the ethanologenic strain of Escherichia coli in BIOTECHNOLOGY LETTERS
  • 1993-09. High cell density cultivation of Escherichia coli W using sucrose as a carbon source in BIOTECHNOLOGY LETTERS
  • 1993-05. A comparative study of glucose conversion to ethanol by Zymomonas mobilis and a recombinant Escherichia coli in BIOTECHNOLOGY LETTERS
  • 1994-02. Comparison of corn steep liquor with other nutrients in the fermentation of D-Xylose by Pichia stipitis CBS 6054 in BIOTECHNOLOGY LETTERS
  • 1992-03. Effect of acetic acid on xylose conversion to ethanol by genetically engineeredE. coli in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02941710

    DOI

    http://dx.doi.org/10.1007/bf02941710

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1011969404

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8669903


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chimera", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cost-Benefit Analysis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Culture Media", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Escherichia coli", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Ethanol", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fermentation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Engineering", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hydrogen-Ion Concentration", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Industrial Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Xylose", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Department of Biochemistry, University of Toronto, M5S 1A8, Toronto, Ontario, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lawford", 
            "givenName": "Hugh G.", 
            "id": "sg:person.01311205507.51", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01311205507.51"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Department of Biochemistry, University of Toronto, M5S 1A8, Toronto, Ontario, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rousseau", 
            "givenName": "Joyce D.", 
            "id": "sg:person.015662325663.16", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015662325663.16"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1021/bp00029a017", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002547038"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01029153", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005063338", 
              "https://doi.org/10.1007/bf01029153"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02933423", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009367803", 
              "https://doi.org/10.1007/bf02933423"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02933423", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009367803", 
              "https://doi.org/10.1007/bf02933423"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00129326", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012539253", 
              "https://doi.org/10.1007/bf00129326"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00129326", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012539253", 
              "https://doi.org/10.1007/bf00129326"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bfb0007858", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013573751", 
              "https://doi.org/10.1007/bfb0007858"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0141-0229(88)90001-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016628925"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0141-0229(88)90001-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016628925"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.251.4999.1318", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022014021"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01021673", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022021624", 
              "https://doi.org/10.1007/bf01021673"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02941709", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023284376", 
              "https://doi.org/10.1007/bf02941709"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02941709", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023284376", 
              "https://doi.org/10.1007/bf02941709"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02922603", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029697110", 
              "https://doi.org/10.1007/bf02922603"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02922603", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029697110", 
              "https://doi.org/10.1007/bf02922603"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.260380311", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030638137"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02920545", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038895016", 
              "https://doi.org/10.1007/bf02920545"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02920545", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038895016", 
              "https://doi.org/10.1007/bf02920545"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00134626", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040302723", 
              "https://doi.org/10.1007/bf00134626"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00134626", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040302723", 
              "https://doi.org/10.1007/bf00134626"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00131766", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040872388", 
              "https://doi.org/10.1007/bf00131766"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00131766", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040872388", 
              "https://doi.org/10.1007/bf00131766"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02920291", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041356063", 
              "https://doi.org/10.1007/bf02920291"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02920291", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041356063", 
              "https://doi.org/10.1007/bf02920291"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02933445", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044607558", 
              "https://doi.org/10.1007/bf02933445"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02933445", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044607558", 
              "https://doi.org/10.1007/bf02933445"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02919028", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049669980", 
              "https://doi.org/10.1007/bf02919028"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02919028", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049669980", 
              "https://doi.org/10.1007/bf02919028"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01025816", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051337379", 
              "https://doi.org/10.1007/bf01025816"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02936498", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052256722", 
              "https://doi.org/10.1007/bf02936498"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02936498", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052256722", 
              "https://doi.org/10.1007/bf02936498"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077157522", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077357078", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1078262595", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1078792257", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1080716887", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082595939", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bk-1994-0566.ch021", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1089359884"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1996-03", 
        "datePublishedReg": "1996-03-01", 
        "description": "This article describes a systematic study of the nutritional requirements of a patented recombinant ethanologenic Escherichia coli (11303:pLO1297) and provides cost-effective formulations that are compatible with the production of fuel ethanol in fermentations of lignocellulosic prehydrolysate characterized by high xylose conversion efficiency. A complex and nutrient-rich laboratory medium, Luria broth (LB), provided the benchmark with respect to fermentation performance standard. Xylose fermentation performance was assessed in terms of the target values for operational process parameters established by the US National Renewable Energy Laboratory (NREL)-final ethanol concentration (25 g/L), xylose-to-ethanol conversion efficiency (90%), and volumetric productivity (0.52 g/L.h). Biomass prehydrolysates that are rich in xylose also contain acetic acid, and in anticipation of a need to reduce acetic acid toxicity, the fermentors were operated with a pH control set-point of 7.0 Growth and fermentation in the minimal defined salts (DS) medium was only about 15% compared to the reference medium. Amendment of the minimal medium containing 6 wt% xylose with both vitamins and amino acids resulted in improved growth, but the volume productivity (0.59 g/L.h) was still only about 54% of that with LB (1.1g/L.h). Formulations directed at cost reduction through the use of less expensive commercial complex nutritional supplements were within 90% of the NREL process target with respect to yield and provided a productivity at about 80% of the LB medium, but were not economical. Corn steep liquor (CSL) at about 7-8 g/L was shown to be a complete source of nutritional requirements and supported a fermentation performance approaching that of LB. At a cost of CSL of $50/t(dry wt), the economic impact of using this amount CSL as the sole nutritional supplement in a cellulosic ethanol plant was estimated to be about 4 cents/gal of ethanol.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/bf02941710", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1086169", 
            "issn": [
              "0273-2289", 
              "1559-0291"
            ], 
            "name": "Applied Biochemistry and Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "57-58"
          }
        ], 
        "name": "Studies on nutrient requirements and cost-effective supplements for ethanol production by recombinantE. coli", 
        "pagination": "307", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "39077394013c3a0b1a6908331b1d8fe772ae20d64760d8267e81e67f6354aff5"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "8669903"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "8208561"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf02941710"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1011969404"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf02941710", 
          "https://app.dimensions.ai/details/publication/pub.1011969404"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T14:28", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000373_0000000373/records_13084_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/BF02941710"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf02941710'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf02941710'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf02941710'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf02941710'


     

    This table displays all metadata directly associated to this object as RDF triples.

    203 TRIPLES      21 PREDICATES      65 URIs      31 LITERALS      19 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf02941710 schema:about N00b15db2ed7645bf9069ef9ef83cfb50
    2 N33d2be8387cf46d7a7d49cfbffe91d07
    3 N46862a4cc1f8488995f1616470c905be
    4 N55d079c94fbe4090be7f221565edc94c
    5 N94daf419e5c940eabe5b94fd3c39f533
    6 Nd481dbc4d8404a66b41d569345575ec9
    7 Nd763c745fbbd4099b83c24a1e8d3892e
    8 Ne4139c20fae04bfe8d2da6374a6e16af
    9 Ne871a17c2e974776ab822219656b9ded
    10 Nfe5f15321c2740f8938a33924e41ab1b
    11 anzsrc-for:06
    12 anzsrc-for:0601
    13 schema:author Nd1a42494f66747df8bdc0b831ba2db46
    14 schema:citation sg:pub.10.1007/bf00129326
    15 sg:pub.10.1007/bf00131766
    16 sg:pub.10.1007/bf00134626
    17 sg:pub.10.1007/bf01021673
    18 sg:pub.10.1007/bf01025816
    19 sg:pub.10.1007/bf01029153
    20 sg:pub.10.1007/bf02919028
    21 sg:pub.10.1007/bf02920291
    22 sg:pub.10.1007/bf02920545
    23 sg:pub.10.1007/bf02922603
    24 sg:pub.10.1007/bf02933423
    25 sg:pub.10.1007/bf02933445
    26 sg:pub.10.1007/bf02936498
    27 sg:pub.10.1007/bf02941709
    28 sg:pub.10.1007/bfb0007858
    29 https://app.dimensions.ai/details/publication/pub.1077157522
    30 https://app.dimensions.ai/details/publication/pub.1077357078
    31 https://app.dimensions.ai/details/publication/pub.1078262595
    32 https://app.dimensions.ai/details/publication/pub.1078792257
    33 https://app.dimensions.ai/details/publication/pub.1080716887
    34 https://app.dimensions.ai/details/publication/pub.1082595939
    35 https://doi.org/10.1002/bit.260380311
    36 https://doi.org/10.1016/0141-0229(88)90001-4
    37 https://doi.org/10.1021/bk-1994-0566.ch021
    38 https://doi.org/10.1021/bp00029a017
    39 https://doi.org/10.1126/science.251.4999.1318
    40 schema:datePublished 1996-03
    41 schema:datePublishedReg 1996-03-01
    42 schema:description This article describes a systematic study of the nutritional requirements of a patented recombinant ethanologenic Escherichia coli (11303:pLO1297) and provides cost-effective formulations that are compatible with the production of fuel ethanol in fermentations of lignocellulosic prehydrolysate characterized by high xylose conversion efficiency. A complex and nutrient-rich laboratory medium, Luria broth (LB), provided the benchmark with respect to fermentation performance standard. Xylose fermentation performance was assessed in terms of the target values for operational process parameters established by the US National Renewable Energy Laboratory (NREL)-final ethanol concentration (25 g/L), xylose-to-ethanol conversion efficiency (90%), and volumetric productivity (0.52 g/L.h). Biomass prehydrolysates that are rich in xylose also contain acetic acid, and in anticipation of a need to reduce acetic acid toxicity, the fermentors were operated with a pH control set-point of 7.0 Growth and fermentation in the minimal defined salts (DS) medium was only about 15% compared to the reference medium. Amendment of the minimal medium containing 6 wt% xylose with both vitamins and amino acids resulted in improved growth, but the volume productivity (0.59 g/L.h) was still only about 54% of that with LB (1.1g/L.h). Formulations directed at cost reduction through the use of less expensive commercial complex nutritional supplements were within 90% of the NREL process target with respect to yield and provided a productivity at about 80% of the LB medium, but were not economical. Corn steep liquor (CSL) at about 7-8 g/L was shown to be a complete source of nutritional requirements and supported a fermentation performance approaching that of LB. At a cost of CSL of $50/t(dry wt), the economic impact of using this amount CSL as the sole nutritional supplement in a cellulosic ethanol plant was estimated to be about 4 cents/gal of ethanol.
    43 schema:genre research_article
    44 schema:inLanguage en
    45 schema:isAccessibleForFree false
    46 schema:isPartOf N137597a72dcc4ff5be6231ce79331527
    47 Ndb24b75bb7b94fd29fec90bdb90ea116
    48 sg:journal.1086169
    49 schema:name Studies on nutrient requirements and cost-effective supplements for ethanol production by recombinantE. coli
    50 schema:pagination 307
    51 schema:productId N0fc665300ad343ceb9a84ceb37925a59
    52 N3ba8dc35f33a495db7657d140a171b22
    53 N846b971927d1427e8a879ec7e847d60c
    54 N913bc2059da54b7293f91d3d8c8aa119
    55 Nfc7915ee054442ecac582142fc0781be
    56 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011969404
    57 https://doi.org/10.1007/bf02941710
    58 schema:sdDatePublished 2019-04-11T14:28
    59 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    60 schema:sdPublisher N2ae07d03652b489f8709e356ce7c83fe
    61 schema:url http://link.springer.com/10.1007/BF02941710
    62 sgo:license sg:explorer/license/
    63 sgo:sdDataset articles
    64 rdf:type schema:ScholarlyArticle
    65 N00b15db2ed7645bf9069ef9ef83cfb50 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    66 schema:name Cost-Benefit Analysis
    67 rdf:type schema:DefinedTerm
    68 N0fc665300ad343ceb9a84ceb37925a59 schema:name readcube_id
    69 schema:value 39077394013c3a0b1a6908331b1d8fe772ae20d64760d8267e81e67f6354aff5
    70 rdf:type schema:PropertyValue
    71 N137597a72dcc4ff5be6231ce79331527 schema:volumeNumber 57-58
    72 rdf:type schema:PublicationVolume
    73 N2ae07d03652b489f8709e356ce7c83fe schema:name Springer Nature - SN SciGraph project
    74 rdf:type schema:Organization
    75 N33d2be8387cf46d7a7d49cfbffe91d07 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    76 schema:name Culture Media
    77 rdf:type schema:DefinedTerm
    78 N3ba8dc35f33a495db7657d140a171b22 schema:name pubmed_id
    79 schema:value 8669903
    80 rdf:type schema:PropertyValue
    81 N46862a4cc1f8488995f1616470c905be schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    82 schema:name Hydrogen-Ion Concentration
    83 rdf:type schema:DefinedTerm
    84 N543b821f4c2a458080785448e7fa310f rdf:first sg:person.015662325663.16
    85 rdf:rest rdf:nil
    86 N55d079c94fbe4090be7f221565edc94c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    87 schema:name Industrial Microbiology
    88 rdf:type schema:DefinedTerm
    89 N846b971927d1427e8a879ec7e847d60c schema:name dimensions_id
    90 schema:value pub.1011969404
    91 rdf:type schema:PropertyValue
    92 N913bc2059da54b7293f91d3d8c8aa119 schema:name doi
    93 schema:value 10.1007/bf02941710
    94 rdf:type schema:PropertyValue
    95 N94daf419e5c940eabe5b94fd3c39f533 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name Escherichia coli
    97 rdf:type schema:DefinedTerm
    98 Nd1a42494f66747df8bdc0b831ba2db46 rdf:first sg:person.01311205507.51
    99 rdf:rest N543b821f4c2a458080785448e7fa310f
    100 Nd481dbc4d8404a66b41d569345575ec9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    101 schema:name Ethanol
    102 rdf:type schema:DefinedTerm
    103 Nd763c745fbbd4099b83c24a1e8d3892e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    104 schema:name Chimera
    105 rdf:type schema:DefinedTerm
    106 Ndb24b75bb7b94fd29fec90bdb90ea116 schema:issueNumber 1
    107 rdf:type schema:PublicationIssue
    108 Ne4139c20fae04bfe8d2da6374a6e16af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    109 schema:name Xylose
    110 rdf:type schema:DefinedTerm
    111 Ne871a17c2e974776ab822219656b9ded schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    112 schema:name Fermentation
    113 rdf:type schema:DefinedTerm
    114 Nfc7915ee054442ecac582142fc0781be schema:name nlm_unique_id
    115 schema:value 8208561
    116 rdf:type schema:PropertyValue
    117 Nfe5f15321c2740f8938a33924e41ab1b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    118 schema:name Genetic Engineering
    119 rdf:type schema:DefinedTerm
    120 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    121 schema:name Biological Sciences
    122 rdf:type schema:DefinedTerm
    123 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    124 schema:name Biochemistry and Cell Biology
    125 rdf:type schema:DefinedTerm
    126 sg:journal.1086169 schema:issn 0273-2289
    127 1559-0291
    128 schema:name Applied Biochemistry and Biotechnology
    129 rdf:type schema:Periodical
    130 sg:person.01311205507.51 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    131 schema:familyName Lawford
    132 schema:givenName Hugh G.
    133 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01311205507.51
    134 rdf:type schema:Person
    135 sg:person.015662325663.16 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    136 schema:familyName Rousseau
    137 schema:givenName Joyce D.
    138 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015662325663.16
    139 rdf:type schema:Person
    140 sg:pub.10.1007/bf00129326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012539253
    141 https://doi.org/10.1007/bf00129326
    142 rdf:type schema:CreativeWork
    143 sg:pub.10.1007/bf00131766 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040872388
    144 https://doi.org/10.1007/bf00131766
    145 rdf:type schema:CreativeWork
    146 sg:pub.10.1007/bf00134626 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040302723
    147 https://doi.org/10.1007/bf00134626
    148 rdf:type schema:CreativeWork
    149 sg:pub.10.1007/bf01021673 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022021624
    150 https://doi.org/10.1007/bf01021673
    151 rdf:type schema:CreativeWork
    152 sg:pub.10.1007/bf01025816 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051337379
    153 https://doi.org/10.1007/bf01025816
    154 rdf:type schema:CreativeWork
    155 sg:pub.10.1007/bf01029153 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005063338
    156 https://doi.org/10.1007/bf01029153
    157 rdf:type schema:CreativeWork
    158 sg:pub.10.1007/bf02919028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049669980
    159 https://doi.org/10.1007/bf02919028
    160 rdf:type schema:CreativeWork
    161 sg:pub.10.1007/bf02920291 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041356063
    162 https://doi.org/10.1007/bf02920291
    163 rdf:type schema:CreativeWork
    164 sg:pub.10.1007/bf02920545 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038895016
    165 https://doi.org/10.1007/bf02920545
    166 rdf:type schema:CreativeWork
    167 sg:pub.10.1007/bf02922603 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029697110
    168 https://doi.org/10.1007/bf02922603
    169 rdf:type schema:CreativeWork
    170 sg:pub.10.1007/bf02933423 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009367803
    171 https://doi.org/10.1007/bf02933423
    172 rdf:type schema:CreativeWork
    173 sg:pub.10.1007/bf02933445 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044607558
    174 https://doi.org/10.1007/bf02933445
    175 rdf:type schema:CreativeWork
    176 sg:pub.10.1007/bf02936498 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052256722
    177 https://doi.org/10.1007/bf02936498
    178 rdf:type schema:CreativeWork
    179 sg:pub.10.1007/bf02941709 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023284376
    180 https://doi.org/10.1007/bf02941709
    181 rdf:type schema:CreativeWork
    182 sg:pub.10.1007/bfb0007858 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013573751
    183 https://doi.org/10.1007/bfb0007858
    184 rdf:type schema:CreativeWork
    185 https://app.dimensions.ai/details/publication/pub.1077157522 schema:CreativeWork
    186 https://app.dimensions.ai/details/publication/pub.1077357078 schema:CreativeWork
    187 https://app.dimensions.ai/details/publication/pub.1078262595 schema:CreativeWork
    188 https://app.dimensions.ai/details/publication/pub.1078792257 schema:CreativeWork
    189 https://app.dimensions.ai/details/publication/pub.1080716887 schema:CreativeWork
    190 https://app.dimensions.ai/details/publication/pub.1082595939 schema:CreativeWork
    191 https://doi.org/10.1002/bit.260380311 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030638137
    192 rdf:type schema:CreativeWork
    193 https://doi.org/10.1016/0141-0229(88)90001-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016628925
    194 rdf:type schema:CreativeWork
    195 https://doi.org/10.1021/bk-1994-0566.ch021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1089359884
    196 rdf:type schema:CreativeWork
    197 https://doi.org/10.1021/bp00029a017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002547038
    198 rdf:type schema:CreativeWork
    199 https://doi.org/10.1126/science.251.4999.1318 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022014021
    200 rdf:type schema:CreativeWork
    201 https://www.grid.ac/institutes/grid.17063.33 schema:alternateName University of Toronto
    202 schema:name Department of Biochemistry, University of Toronto, M5S 1A8, Toronto, Ontario, Canada
    203 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...