Manipulation of microalgal lipid production using genetic engineering View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-03

AUTHORS

Terri G. Dunahay, Eric E. Jarvis, Sonja S. Dais, Paul G. Roessler

ABSTRACT

Genetic transformation of two species of diatoms has been accomplished by introducing chimeric plasmid vectors containing a bacterial antibiotic resistance gene driven by regulatory sequences from the acetyl-CoA carboxylase (ACCase) gene from the diatomCyclotella cryptica. The recombinant DNA integrated into one or more random sites within the algal genome and the foreign protein was produced by the algal transformants. This is the first report of genetic transformation of any chlorophyll c-containing microalgal strain. We are using this system to introduce additional copies of the ACCase gene into diatoms in an attempt to manipulate lipid accumulation in transformed strains. More... »

PAGES

223

Journal

TITLE

Applied Biochemistry and Biotechnology

ISSUE

1

VOLUME

57-58

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02941703

    DOI

    http://dx.doi.org/10.1007/bf02941703

    DIMENSIONS

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