Cloning and characterization ofMannheimia succiniciproducens MBEL55E phosphoenolpyruvate carboxykinase (pckA) gene View Full Text


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Article Info

DATE

2002-04

AUTHORS

Pyung Cheon Lee, Sang Yup Lee, Soon Ho Hong, Ho Nam Chang

ABSTRACT

ApckA gene encoding phosphoenolpyruvate carboxykinase (PEPCK) was cloned and sequenced from the succinic acid producing bacteriumMannheimia succiniciproducens MBEL55E. The gene encoded a 538 residue polypeptide with a calculated molecular mass of 58.8 kDa and a calculated pI of 5.03. The deduced amino acid sequence of theM. succiniciproducens MBEL55E PEPCK was similar to those of all known ATP-dependent PEPCKs. More... »

PAGES

95

Journal

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