Production of cyclodextrin homologues using aqueous two-phase system View Full Text


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Article Info

DATE

1997-12

AUTHORS

Woo-Jin Chang, Yoon-Mo Koo, Sung-Sik Park

ABSTRACT

Cyclodextrin homologues (CDs), produced by cyclodextrin glycosyltransferase (CGTase), were simultaneously partitioned in aqueous two-phase system (ATPS). Partition coefficients of CDs were measured in PEG/salt and PEG/dextran systems. Phosphate, citrate, sulfate were tested as salt. ATPS of PEG/salt and PEG/dextran had the partition coefficients of the CDs, larger than unity. However, PEG/dextran system was observed better than PEG/salt as CGTase activity decreased sharply with salt concentration. Enzymatic reaction occurred mainly in PEG-rich bottom phase because of the low partition coefficient of CGTase. The resulting CDs transferred to the PEG-rich top phase, obeying the diffusional partition. In the ATPS of 7% PEG (M.W. 20,000) and 9% dextran (M.W. 40,000), 7 mg/ml of CDs were obtained in top phase at 4.5 hours. More... »

PAGES

97-100

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Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02932333

DOI

http://dx.doi.org/10.1007/bf02932333

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1004041339


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