Quantitative analysis of oligosaccharide structure of glycoproteins View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-04

AUTHORS

Kern Hee Chang, Tamao Endo, Jung Hoe Kim

ABSTRACT

A sensitive and quantitative method for the structural analysis of oligosaccharide was established for the glycoform analysis of glycoproteins. In this study,N-linked oligosaccharides of human IgG and bovine transferrin were analyzed for the evaluation of the method. Carbohydrate moiety of glycoprotein was released by hydrazinolysis and purified by paper chromatography. The oligosaccharides were labeled with a fluorescent dye, 2-aminobenzamide, for the enhancement of detection sensitivity. Sialylated (acidic) oligosaccharides were separated from neutral oligosaccharide by employing a strong anion-exchange column (MonoO) followed by the treatment with sialidase. Enzymatically desialyated fractions and neutral fractions of oligosaccharides were applied to normal-phase HPLC to resolve the peaks according to glucose unit (GU). The structure of separated molecules was further determined by sequential digestion with exoglycosidases. As a result, disialylated biantennary complextype oligo saccharide was found to be a major sugar chain in bovine transferrin (63%). In human IgG, core fucosylated asialobiantennary complex oligosaccharides were dominant. These results coincided well with reported results. More... »

PAGES

136

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02931885

DOI

http://dx.doi.org/10.1007/bf02931885

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019274249


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Korea Advanced Institute of Science and Technology", 
          "id": "https://www.grid.ac/institutes/grid.37172.30", 
          "name": [
            "Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Kusung dong, Yusung gu, 305-701, Taejon, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chang", 
        "givenName": "Kern Hee", 
        "id": "sg:person.012456547353.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012456547353.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tokyo Metropolitan Institute of Gerontology", 
          "id": "https://www.grid.ac/institutes/grid.420122.7", 
          "name": [
            "Department of Glycobiology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, 173-0015, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Endo", 
        "givenName": "Tamao", 
        "id": "sg:person.014313476452.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014313476452.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Korea Advanced Institute of Science and Technology", 
          "id": "https://www.grid.ac/institutes/grid.37172.30", 
          "name": [
            "Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Kusung dong, Yusung gu, 305-701, Taejon, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Jung Hoe", 
        "id": "sg:person.01300137557.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300137557.41"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.86.20.7819", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005173086"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02932482", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006817131", 
          "https://doi.org/10.1007/bf02932482"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02932482", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006817131", 
          "https://doi.org/10.1007/bf02932482"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj1450263", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009425486"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj1450263", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009425486"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/abio.1995.1468", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019042209"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/abbi.1993.1569", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034217344"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/abio.1996.0351", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040268046"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.bi.49.070180.002041", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046956370"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0076-6879(82)83019-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049963381"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078739176", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078997863", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081972815", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2000-04", 
    "datePublishedReg": "2000-04-01", 
    "description": "A sensitive and quantitative method for the structural analysis of oligosaccharide was established for the glycoform analysis of glycoproteins. In this study,N-linked oligosaccharides of human IgG and bovine transferrin were analyzed for the evaluation of the method. Carbohydrate moiety of glycoprotein was released by hydrazinolysis and purified by paper chromatography. The oligosaccharides were labeled with a fluorescent dye, 2-aminobenzamide, for the enhancement of detection sensitivity. Sialylated (acidic) oligosaccharides were separated from neutral oligosaccharide by employing a strong anion-exchange column (MonoO) followed by the treatment with sialidase. Enzymatically desialyated fractions and neutral fractions of oligosaccharides were applied to normal-phase HPLC to resolve the peaks according to glucose unit (GU). The structure of separated molecules was further determined by sequential digestion with exoglycosidases. As a result, disialylated biantennary complextype oligo saccharide was found to be a major sugar chain in bovine transferrin (63%). In human IgG, core fucosylated asialobiantennary complex oligosaccharides were dominant. These results coincided well with reported results.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf02931885", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1036335", 
        "issn": [
          "1226-8372", 
          "1976-3816"
        ], 
        "name": "Biotechnology and Bioprocess Engineering", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "5"
      }
    ], 
    "name": "Quantitative analysis of oligosaccharide structure of glycoproteins", 
    "pagination": "136", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "5bd5cf686c29e6e01955fc64bdd654a9cce991f14b86d2b01f3d967a45453e56"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf02931885"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1019274249"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf02931885", 
      "https://app.dimensions.ai/details/publication/pub.1019274249"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:47", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130792_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2FBF02931885"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf02931885'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf02931885'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf02931885'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf02931885'


 

This table displays all metadata directly associated to this object as RDF triples.

109 TRIPLES      21 PREDICATES      38 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf02931885 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author Na71aa58f060d49a594394b83989edd7c
4 schema:citation sg:pub.10.1007/bf02932482
5 https://app.dimensions.ai/details/publication/pub.1078739176
6 https://app.dimensions.ai/details/publication/pub.1078997863
7 https://app.dimensions.ai/details/publication/pub.1081972815
8 https://doi.org/10.1006/abbi.1993.1569
9 https://doi.org/10.1006/abio.1995.1468
10 https://doi.org/10.1006/abio.1996.0351
11 https://doi.org/10.1016/0076-6879(82)83019-x
12 https://doi.org/10.1042/bj1450263
13 https://doi.org/10.1073/pnas.86.20.7819
14 https://doi.org/10.1146/annurev.bi.49.070180.002041
15 schema:datePublished 2000-04
16 schema:datePublishedReg 2000-04-01
17 schema:description A sensitive and quantitative method for the structural analysis of oligosaccharide was established for the glycoform analysis of glycoproteins. In this study,N-linked oligosaccharides of human IgG and bovine transferrin were analyzed for the evaluation of the method. Carbohydrate moiety of glycoprotein was released by hydrazinolysis and purified by paper chromatography. The oligosaccharides were labeled with a fluorescent dye, 2-aminobenzamide, for the enhancement of detection sensitivity. Sialylated (acidic) oligosaccharides were separated from neutral oligosaccharide by employing a strong anion-exchange column (MonoO) followed by the treatment with sialidase. Enzymatically desialyated fractions and neutral fractions of oligosaccharides were applied to normal-phase HPLC to resolve the peaks according to glucose unit (GU). The structure of separated molecules was further determined by sequential digestion with exoglycosidases. As a result, disialylated biantennary complextype oligo saccharide was found to be a major sugar chain in bovine transferrin (63%). In human IgG, core fucosylated asialobiantennary complex oligosaccharides were dominant. These results coincided well with reported results.
18 schema:genre research_article
19 schema:inLanguage en
20 schema:isAccessibleForFree false
21 schema:isPartOf N5810e85aa0e843d4866c34ce8516483b
22 Nf20dd6d7513e4011a909fe77d1b76a06
23 sg:journal.1036335
24 schema:name Quantitative analysis of oligosaccharide structure of glycoproteins
25 schema:pagination 136
26 schema:productId N0edb4e3e33444d22af1d239036b966a5
27 N1dccda80f3a748e6ad41fabb3ba505fa
28 Ne2dace1651594b2185181bbd94a84025
29 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019274249
30 https://doi.org/10.1007/bf02931885
31 schema:sdDatePublished 2019-04-11T13:47
32 schema:sdLicense https://scigraph.springernature.com/explorer/license/
33 schema:sdPublisher N3b3688bf19834b4a908cb49a18b45887
34 schema:url http://link.springer.com/10.1007%2FBF02931885
35 sgo:license sg:explorer/license/
36 sgo:sdDataset articles
37 rdf:type schema:ScholarlyArticle
38 N0edb4e3e33444d22af1d239036b966a5 schema:name doi
39 schema:value 10.1007/bf02931885
40 rdf:type schema:PropertyValue
41 N1dccda80f3a748e6ad41fabb3ba505fa schema:name dimensions_id
42 schema:value pub.1019274249
43 rdf:type schema:PropertyValue
44 N29ad7e8204f64891bbc5445e44c829c2 rdf:first sg:person.014313476452.11
45 rdf:rest N9ff0ac4054e24fdd9224828435555e52
46 N3b3688bf19834b4a908cb49a18b45887 schema:name Springer Nature - SN SciGraph project
47 rdf:type schema:Organization
48 N5810e85aa0e843d4866c34ce8516483b schema:volumeNumber 5
49 rdf:type schema:PublicationVolume
50 N9ff0ac4054e24fdd9224828435555e52 rdf:first sg:person.01300137557.41
51 rdf:rest rdf:nil
52 Na71aa58f060d49a594394b83989edd7c rdf:first sg:person.012456547353.20
53 rdf:rest N29ad7e8204f64891bbc5445e44c829c2
54 Ne2dace1651594b2185181bbd94a84025 schema:name readcube_id
55 schema:value 5bd5cf686c29e6e01955fc64bdd654a9cce991f14b86d2b01f3d967a45453e56
56 rdf:type schema:PropertyValue
57 Nf20dd6d7513e4011a909fe77d1b76a06 schema:issueNumber 2
58 rdf:type schema:PublicationIssue
59 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
60 schema:name Biological Sciences
61 rdf:type schema:DefinedTerm
62 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
63 schema:name Biochemistry and Cell Biology
64 rdf:type schema:DefinedTerm
65 sg:journal.1036335 schema:issn 1226-8372
66 1976-3816
67 schema:name Biotechnology and Bioprocess Engineering
68 rdf:type schema:Periodical
69 sg:person.012456547353.20 schema:affiliation https://www.grid.ac/institutes/grid.37172.30
70 schema:familyName Chang
71 schema:givenName Kern Hee
72 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012456547353.20
73 rdf:type schema:Person
74 sg:person.01300137557.41 schema:affiliation https://www.grid.ac/institutes/grid.37172.30
75 schema:familyName Kim
76 schema:givenName Jung Hoe
77 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300137557.41
78 rdf:type schema:Person
79 sg:person.014313476452.11 schema:affiliation https://www.grid.ac/institutes/grid.420122.7
80 schema:familyName Endo
81 schema:givenName Tamao
82 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014313476452.11
83 rdf:type schema:Person
84 sg:pub.10.1007/bf02932482 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006817131
85 https://doi.org/10.1007/bf02932482
86 rdf:type schema:CreativeWork
87 https://app.dimensions.ai/details/publication/pub.1078739176 schema:CreativeWork
88 https://app.dimensions.ai/details/publication/pub.1078997863 schema:CreativeWork
89 https://app.dimensions.ai/details/publication/pub.1081972815 schema:CreativeWork
90 https://doi.org/10.1006/abbi.1993.1569 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034217344
91 rdf:type schema:CreativeWork
92 https://doi.org/10.1006/abio.1995.1468 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019042209
93 rdf:type schema:CreativeWork
94 https://doi.org/10.1006/abio.1996.0351 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040268046
95 rdf:type schema:CreativeWork
96 https://doi.org/10.1016/0076-6879(82)83019-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1049963381
97 rdf:type schema:CreativeWork
98 https://doi.org/10.1042/bj1450263 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009425486
99 rdf:type schema:CreativeWork
100 https://doi.org/10.1073/pnas.86.20.7819 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005173086
101 rdf:type schema:CreativeWork
102 https://doi.org/10.1146/annurev.bi.49.070180.002041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046956370
103 rdf:type schema:CreativeWork
104 https://www.grid.ac/institutes/grid.37172.30 schema:alternateName Korea Advanced Institute of Science and Technology
105 schema:name Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Kusung dong, Yusung gu, 305-701, Taejon, Korea
106 rdf:type schema:Organization
107 https://www.grid.ac/institutes/grid.420122.7 schema:alternateName Tokyo Metropolitan Institute of Gerontology
108 schema:name Department of Glycobiology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, 173-0015, Tokyo, Japan
109 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...