Microbiological aspects of determination of trichloroacetic acid in soil View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-03

AUTHORS

M. Matucha, M. Gryndler, H. Uhlířová, K. Fuksová, J. Rohlenová, S. T. Forczek, P. Schroder

ABSTRACT

Soils have been shown to possess a strong microbial trichloroacetic acid (TCA)-degrading activity. High TCA-degradation rate was also observed during soil extraction with water. For correct measurements of TCA levels in soil all TCA-degrading activities have to be inhibited immediately after sampling before analysis. We used rapid freezing of soil samples (optimally in liquid nitrogen) with subsequent storage and slow thawing before analysis as an efficient technique for suppressing the degradation. Frozen soil samples stored overnight at −20 °C and then thawed slowly exhibited very low residual TCA-degrading activity for several hours. Omitting the above procedure could lead to the confusing differences between the TCA levels previously reported in the literature. More... »

PAGES

117

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02931384

DOI

http://dx.doi.org/10.1007/bf02931384

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1015333660

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15227781


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