The effect of fluidic conditions on the continuous-flow bioluminescent detection of ATP in a microfluidic device View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-10

AUTHORS

Thuan-Hieu Tran, Woo-Jin Chang, Young-Bum Kim, Yoon-Mo Koo, Eun-Ki Kim, Joo-Young Yoon, Jinhwan Kim

ABSTRACT

The effect of fluidic conditions on the bioluminescent detection of ATP in a microfluidic device was surveyed using homemade detector system. The bioluminescent reaction of ATP was directly affected by the retention time and flow rates of the solutions in this diffusion-based mixing and reaction system due to the laminar flow in the microchannel. ATP and enzyme solutions were separately injected into the microfluidic device at different flow rates through 3 inlet ports. The ATP solution was injected through the middle port, while the enzyme solution was injected in the two remaining ports. When the ratio of ATP to enzyme solution was fixed, the optimum flow rates of enzyme, ATP, and enzyme solution was 3.5, 8.0, and 3.5 μL/min, respectively. The optimal total flow rate was 15 μL/min. The detection limit for the concentration of ATP at optimal conditions was less than 10−7 M. More... »

PAGES

470

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02931342

DOI

http://dx.doi.org/10.1007/bf02931342

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019907789


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