Ergosterol synthesis and population Analysis of a fed-batch fermentation inSaccharomyces cerevisiae View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-04

AUTHORS

A. Pichová, K. Beran, B. BĚhalová, J. Zajíček

ABSTRACT

Saccharomyces cerevisiae with an increased content of ergosterol or delta 5,7-sterols, growing on a molasses medium with a feed of ethanol and (NH4)2HPO4, was analyzed as to the age of cell population. The analysis was done by centrifugation in a dextran gradient and by a fluorescence-microscopic technique. In the phase of batch fermentation at a mean specific growth rate of 0.22 h-1 daughter cells contained less than 1% ergosterol while the ergosterol content of mother cells depended on the time of cultivation, a maximum level (4%) being found after two generation times. In the fed-batch phase at a mean growth rate of 0.052 h-1, both daughter and mother cells contained about the same amount of ergosterol (4.7-5.5%). Differences between daughter and mother cells are discussed in view of the relationship between the growth rate and the growth cycle. More... »

PAGES

134-140

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02922206

DOI

http://dx.doi.org/10.1007/bf02922206

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019686594

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/3888793


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