A biosensor for L-proline determination by use of immobilized microbial cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-09

AUTHORS

A. L. Simonian, E. I. Rainina, V. I. Lozinsky, I. E. Badalian, G. E. Khachatrian, S. Sh. Tatikian, T. A. Makhlis, S. D. Varfolomeyev

ABSTRACT

A biosensor to quantify L-proline within 10-5–10-3 mole/L concentration is described. ImmobilizedPseudomonas sp. cells grown in a medium containing L-proline as the only source of carbon and nitrogen were used to create the biosensor. The cells oxidized L-proline specifically consuming O2 and did not react with other amino acids and sugars. The change in oxygen concentration was detected with a Clark oxygen membrane electrode. The cells were immobilized by entrapment in polyvinyl alcohol (PVA) cryogel. The resultant biocatalyst had a high mechanical strength and retained its L-proline-oxidizing ability for at least two months. More... »

PAGES

199-210

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02921779

DOI

http://dx.doi.org/10.1007/bf02921779

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1015228617

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1288409


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