Production of l-malic acid via biocatalysis employing wild-type and respiratory-deficient yeasts View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-03

AUTHORS

Xiaohai Wang, C. S. Gong, George T. Tsao

ABSTRACT

The yeast Saccharomyces cerevisiae has been used to efficiently produce L-malic acid from fumaric acid. Fumarase is responsible for the reversible conversion of fumaric and L-malic acids in the TCA cycle. To investigate the function of mitochondrial and cytoplasmic fumarase isoenzymes in L-malic acid bioconversion, a wild-type strain and a cytoplasmic respiratory-deficient mutant devoid of functional mitochondria were employed. The mutant strain, which only contained the cytoplasmic fumarase, was still functional in fumaric acid to L-malic acid bioconversion However, its specific conversion rate was much lower (0.20 g/g.h) than that of the wild-type strain (0.55 g/g.h). More... »

PAGES

845

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02920194

DOI

http://dx.doi.org/10.1007/bf02920194

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042685215

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9627400


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