Characterization of F VIII concentrates produced by two methods incorporating double virus inactivation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-02

AUTHORS

Karmen Branović, Branka Gebauer, Anđa Treščec, Bojan Benko

ABSTRACT

The trend toward the production of high purity factor VIII concentrates for clinical use is still in progress. Although all plasma derivatives must undergo viral inactivation procedures, the possibility of transmission of viral diseases is not completely eliminated. In order to reduce such risk, we have included double virus inactivation in the procedure of factor VIII concentrate production. In a scale-up procedure for isolation of factor VIII cryoprecipitate, two methods were used. The first is based on the chromatographic purification of factor VIII after pasteurization of cryoprecipitate solution and solvent/detergent (S/D) inactivation of viruses. The second is based on multistep precipitation of factor VIII by sodium chloride and glycine. Viral inactivation was performed by combination of S/D treatment and heating of final freeze-dried product 30 min at 100 degrees C. The typical yield of factor VIII activity in the freeze-dried product was about 20% for the first method, and 25-30% for the second. Electrophoretic analyses of both factor VIII preparations by SDS-PAGE and IEF show very low content of contaminant proteins, in accordance with observed 400-650-fold increase of their specific activity over plasma. Both factor VIII products were stable in the liquid state for more than 24 h at room temperature. The final products, after double viral inactivation, are considered to be suitable for clinical evaluations. More... »

PAGES

99-111

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02919392

DOI

http://dx.doi.org/10.1007/bf02919392

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045276019

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9513991


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