Somatic hybrid plants of potato and tomato regenerated from fused protoplasts View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1978-07

AUTHORS

Georg Melchers, Maria D. Sacristán, Anthony A. Holder

ABSTRACT

Mesophyll protoplasts of Lycopersicon esculentum Mill. var. cerasiforme (Dunal) Alef, mutant yellow green 6, Rick and protoplasts of a liquid callus culture of the dihaploid strain HH258 of Solanum tuberosum L. were prepared and many fusion products were visible after the protoplasts were incubated together first in the presence of polyethylene glycol and then with a high Ca2+ ion concentration. The protoplasts were transferred to a rich medium and the resultant calli were cultured. Some calli regenerated normal green shoots which were transferred to soil or grafted onto a tomato stock. The subunit polypeptide pattern of ribulose 1,5-bisphosphate carboxylase prepared from leaf material of four regenerated plants was analyzed by isoelectric focusing. The ribulose bisphosphate carboxylase enzyme oligomer in the four plants contained the small subunit products resulting from the expression of both tomato and potato nuclear genes proving these plants to be somatic hybrids between tomato and potato. In three of the four plants the large subunit polypeptides and hence the functional chloroplast DNA were from tomato whereas in the fourth the large subunit and therefore the chloroplast DNA was derived from potato. The plant material was insufficient to establish the chromosome numbers precisely, however counts close to 50 which is near to the expected 48 were obtained for three of the hybrids whereas in the fourth a number close to 72 was observed. In the absence of a selection system against the potato parent, the analysis of ribulose bisphosphate carboxylase provides a convenient marker to demonstrate the hybrid nature of the plants. More... »

PAGES

203

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02906548

DOI

http://dx.doi.org/10.1007/bf02906548

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1012846344


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Plant Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Max Planck Institute for Biology", 
          "id": "https://www.grid.ac/institutes/grid.419580.1", 
          "name": [
            "Max-Planck-Institut f\u00fcr Biologie, Corrensstrasse 45, D-7400, T\u00fcbingen"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Melchers", 
        "givenName": "Georg", 
        "id": "sg:person.015070757623.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015070757623.38"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institut f\u00fcr Angewandte Genetik der Freien Universit\u00e4t, Albrecht-Thaer-Weg 6, D-1000, Berlin 33"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sacrist\u00e1n", 
        "givenName": "Maria D.", 
        "id": "sg:person.016441477436.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016441477436.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Carlsberg Laboratory", 
          "id": "https://www.grid.ac/institutes/grid.418674.8", 
          "name": [
            "Department of Physiology, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Copenhagen, Valby"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Holder", 
        "givenName": "Anthony A.", 
        "id": "sg:person.01171476745.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01171476745.37"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0003-9861(76)90048-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001231319"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00268121", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002069375", 
          "https://doi.org/10.1007/bf00268121"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00268121", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002069375", 
          "https://doi.org/10.1007/bf00268121"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.74.3.1082", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002466251"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.74.12.5487", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004512076"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/252226a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004726783", 
          "https://doi.org/10.1038/252226a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00380613", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006514198", 
          "https://doi.org/10.1007/bf00380613"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00380613", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006514198", 
          "https://doi.org/10.1007/bf00380613"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00277587", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009237917", 
          "https://doi.org/10.1007/bf00277587"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00277587", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009237917", 
          "https://doi.org/10.1007/bf00277587"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00624737", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009833454", 
          "https://doi.org/10.1007/bf00624737"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/263500a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013359217", 
          "https://doi.org/10.1038/263500a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02906140", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013995291", 
          "https://doi.org/10.1007/bf02906140"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02906140", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013995291", 
          "https://doi.org/10.1007/bf02906140"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00267868", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015698649", 
          "https://doi.org/10.1007/bf00267868"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00267868", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015698649", 
          "https://doi.org/10.1007/bf00267868"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00484459", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016813377", 
          "https://doi.org/10.1007/bf00484459"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00484459", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016813377", 
          "https://doi.org/10.1007/bf00484459"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2795(77)90052-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017454934"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2795(77)90052-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017454934"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/j.1601-5223.1976.tb01545.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019019294", 
          "https://doi.org/10.1111/j.1601-5223.1976.tb01545.x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4211(78)90151-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019566278"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4211(78)90151-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019566278"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00388618", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024300197", 
          "https://doi.org/10.1007/bf00388618"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00388618", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024300197", 
          "https://doi.org/10.1007/bf00388618"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4211(77)90116-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028266837"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.74.8.3193", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030319913"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2787(72)90115-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032678369"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2787(72)90115-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032678369"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00268120", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036028356", 
          "https://doi.org/10.1007/bf00268120"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00268120", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036028356", 
          "https://doi.org/10.1007/bf00268120"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00267405", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037366708", 
          "https://doi.org/10.1007/bf00267405"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00267405", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037366708", 
          "https://doi.org/10.1007/bf00267405"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-9861(78)90167-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039981777"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2787(72)90217-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044275474"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2787(72)90217-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044275474"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00269080", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044812453", 
          "https://doi.org/10.1007/bf00269080"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00269080", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044812453", 
          "https://doi.org/10.1007/bf00269080"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/271420a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047848205", 
          "https://doi.org/10.1038/271420a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00271144", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049230604", 
          "https://doi.org/10.1007/bf00271144"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00271144", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049230604", 
          "https://doi.org/10.1007/bf00271144"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01740889", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052764438", 
          "https://doi.org/10.1007/bf01740889"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01740889", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052764438", 
          "https://doi.org/10.1007/bf01740889"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1108201", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062451473"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.187.4174.353", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062511016"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.190.4221.1304", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062512924"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/znc-1973-11-1215", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1067583450"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077356546", 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00281321", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078824779", 
          "https://doi.org/10.1007/bf00281321"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00281321", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078824779", 
          "https://doi.org/10.1007/bf00281321"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00273824", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078824795", 
          "https://doi.org/10.1007/bf00273824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00273824", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078824795", 
          "https://doi.org/10.1007/bf00273824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/oxfordjournals.jhered.a108685", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083485379"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0015-3796(17)30861-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084042461"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0015-3796(17)30984-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084042551"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1978-07", 
    "datePublishedReg": "1978-07-01", 
    "description": "Mesophyll protoplasts of Lycopersicon esculentum Mill. var. cerasiforme (Dunal) Alef, mutant yellow green 6, Rick and protoplasts of a liquid callus culture of the dihaploid strain HH258 of Solanum tuberosum L. were prepared and many fusion products were visible after the protoplasts were incubated together first in the presence of polyethylene glycol and then with a high Ca2+ ion concentration. The protoplasts were transferred to a rich medium and the resultant calli were cultured. Some calli regenerated normal green shoots which were transferred to soil or grafted onto a tomato stock. The subunit polypeptide pattern of ribulose 1,5-bisphosphate carboxylase prepared from leaf material of four regenerated plants was analyzed by isoelectric focusing. The ribulose bisphosphate carboxylase enzyme oligomer in the four plants contained the small subunit products resulting from the expression of both tomato and potato nuclear genes proving these plants to be somatic hybrids between tomato and potato. In three of the four plants the large subunit polypeptides and hence the functional chloroplast DNA were from tomato whereas in the fourth the large subunit and therefore the chloroplast DNA was derived from potato. The plant material was insufficient to establish the chromosome numbers precisely, however counts close to 50 which is near to the expected 48 were obtained for three of the hybrids whereas in the fourth a number close to 72 was observed. In the absence of a selection system against the potato parent, the analysis of ribulose bisphosphate carboxylase provides a convenient marker to demonstrate the hybrid nature of the plants.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf02906548", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1019851", 
        "issn": [
          "0105-1938"
        ], 
        "name": "Carlsberg Research Communications", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "43"
      }
    ], 
    "name": "Somatic hybrid plants of potato and tomato regenerated from fused protoplasts", 
    "pagination": "203", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "3d32c6c56de77fb8826f13112aa3efe615ed087610a95fcad907fb0113cb323b"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf02906548"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1012846344"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf02906548", 
      "https://app.dimensions.ai/details/publication/pub.1012846344"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:50", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130797_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2FBF02906548"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf02906548'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf02906548'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf02906548'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf02906548'


 

This table displays all metadata directly associated to this object as RDF triples.

208 TRIPLES      21 PREDICATES      64 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf02906548 schema:about anzsrc-for:06
2 anzsrc-for:0607
3 schema:author N3c3891de12264c6cb1a8372a813689be
4 schema:citation sg:pub.10.1007/bf00267405
5 sg:pub.10.1007/bf00267868
6 sg:pub.10.1007/bf00268120
7 sg:pub.10.1007/bf00268121
8 sg:pub.10.1007/bf00269080
9 sg:pub.10.1007/bf00271144
10 sg:pub.10.1007/bf00273824
11 sg:pub.10.1007/bf00277587
12 sg:pub.10.1007/bf00281321
13 sg:pub.10.1007/bf00380613
14 sg:pub.10.1007/bf00388618
15 sg:pub.10.1007/bf00484459
16 sg:pub.10.1007/bf00624737
17 sg:pub.10.1007/bf01740889
18 sg:pub.10.1007/bf02906140
19 sg:pub.10.1038/252226a0
20 sg:pub.10.1038/263500a0
21 sg:pub.10.1038/271420a0
22 sg:pub.10.1111/j.1601-5223.1976.tb01545.x
23 https://app.dimensions.ai/details/publication/pub.1077356546
24 https://doi.org/10.1016/0003-9861(76)90048-5
25 https://doi.org/10.1016/0003-9861(78)90167-4
26 https://doi.org/10.1016/0005-2787(72)90115-3
27 https://doi.org/10.1016/0005-2787(72)90217-1
28 https://doi.org/10.1016/0005-2795(77)90052-6
29 https://doi.org/10.1016/0304-4211(77)90116-x
30 https://doi.org/10.1016/0304-4211(78)90151-7
31 https://doi.org/10.1016/s0015-3796(17)30861-2
32 https://doi.org/10.1016/s0015-3796(17)30984-8
33 https://doi.org/10.1073/pnas.74.12.5487
34 https://doi.org/10.1073/pnas.74.3.1082
35 https://doi.org/10.1073/pnas.74.8.3193
36 https://doi.org/10.1093/oxfordjournals.jhered.a108685
37 https://doi.org/10.1126/science.1108201
38 https://doi.org/10.1126/science.187.4174.353
39 https://doi.org/10.1126/science.190.4221.1304
40 https://doi.org/10.1515/znc-1973-11-1215
41 schema:datePublished 1978-07
42 schema:datePublishedReg 1978-07-01
43 schema:description Mesophyll protoplasts of Lycopersicon esculentum Mill. var. cerasiforme (Dunal) Alef, mutant yellow green 6, Rick and protoplasts of a liquid callus culture of the dihaploid strain HH258 of Solanum tuberosum L. were prepared and many fusion products were visible after the protoplasts were incubated together first in the presence of polyethylene glycol and then with a high Ca2+ ion concentration. The protoplasts were transferred to a rich medium and the resultant calli were cultured. Some calli regenerated normal green shoots which were transferred to soil or grafted onto a tomato stock. The subunit polypeptide pattern of ribulose 1,5-bisphosphate carboxylase prepared from leaf material of four regenerated plants was analyzed by isoelectric focusing. The ribulose bisphosphate carboxylase enzyme oligomer in the four plants contained the small subunit products resulting from the expression of both tomato and potato nuclear genes proving these plants to be somatic hybrids between tomato and potato. In three of the four plants the large subunit polypeptides and hence the functional chloroplast DNA were from tomato whereas in the fourth the large subunit and therefore the chloroplast DNA was derived from potato. The plant material was insufficient to establish the chromosome numbers precisely, however counts close to 50 which is near to the expected 48 were obtained for three of the hybrids whereas in the fourth a number close to 72 was observed. In the absence of a selection system against the potato parent, the analysis of ribulose bisphosphate carboxylase provides a convenient marker to demonstrate the hybrid nature of the plants.
44 schema:genre research_article
45 schema:inLanguage en
46 schema:isAccessibleForFree true
47 schema:isPartOf N42a6c99dcb844266b6f34eded13fccaa
48 N99f42775630449bbadea2650a1c495c2
49 sg:journal.1019851
50 schema:name Somatic hybrid plants of potato and tomato regenerated from fused protoplasts
51 schema:pagination 203
52 schema:productId N1d01c33514af4072a69d651e0323d033
53 N4a5e501611754c719cedd6367f4cee7d
54 Ne10d634848634cc2bbb0369cd8202bb3
55 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012846344
56 https://doi.org/10.1007/bf02906548
57 schema:sdDatePublished 2019-04-11T13:50
58 schema:sdLicense https://scigraph.springernature.com/explorer/license/
59 schema:sdPublisher N5e585d5cf8184b9d833b5907da80216f
60 schema:url http://link.springer.com/10.1007%2FBF02906548
61 sgo:license sg:explorer/license/
62 sgo:sdDataset articles
63 rdf:type schema:ScholarlyArticle
64 N114157d20fd849c4a8e975f29ba958f3 rdf:first sg:person.01171476745.37
65 rdf:rest rdf:nil
66 N1d01c33514af4072a69d651e0323d033 schema:name readcube_id
67 schema:value 3d32c6c56de77fb8826f13112aa3efe615ed087610a95fcad907fb0113cb323b
68 rdf:type schema:PropertyValue
69 N3c3891de12264c6cb1a8372a813689be rdf:first sg:person.015070757623.38
70 rdf:rest N6e3a74d135dd4bb7ae6edab93d55ea9f
71 N42a6c99dcb844266b6f34eded13fccaa schema:issueNumber 4
72 rdf:type schema:PublicationIssue
73 N4a5e501611754c719cedd6367f4cee7d schema:name dimensions_id
74 schema:value pub.1012846344
75 rdf:type schema:PropertyValue
76 N4c531249d97841b7b17638fdb298955e schema:name Institut für Angewandte Genetik der Freien Universität, Albrecht-Thaer-Weg 6, D-1000, Berlin 33
77 rdf:type schema:Organization
78 N5e585d5cf8184b9d833b5907da80216f schema:name Springer Nature - SN SciGraph project
79 rdf:type schema:Organization
80 N6e3a74d135dd4bb7ae6edab93d55ea9f rdf:first sg:person.016441477436.10
81 rdf:rest N114157d20fd849c4a8e975f29ba958f3
82 N99f42775630449bbadea2650a1c495c2 schema:volumeNumber 43
83 rdf:type schema:PublicationVolume
84 Ne10d634848634cc2bbb0369cd8202bb3 schema:name doi
85 schema:value 10.1007/bf02906548
86 rdf:type schema:PropertyValue
87 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
88 schema:name Biological Sciences
89 rdf:type schema:DefinedTerm
90 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
91 schema:name Plant Biology
92 rdf:type schema:DefinedTerm
93 sg:journal.1019851 schema:issn 0105-1938
94 schema:name Carlsberg Research Communications
95 rdf:type schema:Periodical
96 sg:person.01171476745.37 schema:affiliation https://www.grid.ac/institutes/grid.418674.8
97 schema:familyName Holder
98 schema:givenName Anthony A.
99 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01171476745.37
100 rdf:type schema:Person
101 sg:person.015070757623.38 schema:affiliation https://www.grid.ac/institutes/grid.419580.1
102 schema:familyName Melchers
103 schema:givenName Georg
104 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015070757623.38
105 rdf:type schema:Person
106 sg:person.016441477436.10 schema:affiliation N4c531249d97841b7b17638fdb298955e
107 schema:familyName Sacristán
108 schema:givenName Maria D.
109 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016441477436.10
110 rdf:type schema:Person
111 sg:pub.10.1007/bf00267405 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037366708
112 https://doi.org/10.1007/bf00267405
113 rdf:type schema:CreativeWork
114 sg:pub.10.1007/bf00267868 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015698649
115 https://doi.org/10.1007/bf00267868
116 rdf:type schema:CreativeWork
117 sg:pub.10.1007/bf00268120 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036028356
118 https://doi.org/10.1007/bf00268120
119 rdf:type schema:CreativeWork
120 sg:pub.10.1007/bf00268121 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002069375
121 https://doi.org/10.1007/bf00268121
122 rdf:type schema:CreativeWork
123 sg:pub.10.1007/bf00269080 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044812453
124 https://doi.org/10.1007/bf00269080
125 rdf:type schema:CreativeWork
126 sg:pub.10.1007/bf00271144 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049230604
127 https://doi.org/10.1007/bf00271144
128 rdf:type schema:CreativeWork
129 sg:pub.10.1007/bf00273824 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078824795
130 https://doi.org/10.1007/bf00273824
131 rdf:type schema:CreativeWork
132 sg:pub.10.1007/bf00277587 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009237917
133 https://doi.org/10.1007/bf00277587
134 rdf:type schema:CreativeWork
135 sg:pub.10.1007/bf00281321 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078824779
136 https://doi.org/10.1007/bf00281321
137 rdf:type schema:CreativeWork
138 sg:pub.10.1007/bf00380613 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006514198
139 https://doi.org/10.1007/bf00380613
140 rdf:type schema:CreativeWork
141 sg:pub.10.1007/bf00388618 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024300197
142 https://doi.org/10.1007/bf00388618
143 rdf:type schema:CreativeWork
144 sg:pub.10.1007/bf00484459 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016813377
145 https://doi.org/10.1007/bf00484459
146 rdf:type schema:CreativeWork
147 sg:pub.10.1007/bf00624737 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009833454
148 https://doi.org/10.1007/bf00624737
149 rdf:type schema:CreativeWork
150 sg:pub.10.1007/bf01740889 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052764438
151 https://doi.org/10.1007/bf01740889
152 rdf:type schema:CreativeWork
153 sg:pub.10.1007/bf02906140 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013995291
154 https://doi.org/10.1007/bf02906140
155 rdf:type schema:CreativeWork
156 sg:pub.10.1038/252226a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004726783
157 https://doi.org/10.1038/252226a0
158 rdf:type schema:CreativeWork
159 sg:pub.10.1038/263500a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013359217
160 https://doi.org/10.1038/263500a0
161 rdf:type schema:CreativeWork
162 sg:pub.10.1038/271420a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047848205
163 https://doi.org/10.1038/271420a0
164 rdf:type schema:CreativeWork
165 sg:pub.10.1111/j.1601-5223.1976.tb01545.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1019019294
166 https://doi.org/10.1111/j.1601-5223.1976.tb01545.x
167 rdf:type schema:CreativeWork
168 https://app.dimensions.ai/details/publication/pub.1077356546 schema:CreativeWork
169 https://doi.org/10.1016/0003-9861(76)90048-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001231319
170 rdf:type schema:CreativeWork
171 https://doi.org/10.1016/0003-9861(78)90167-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039981777
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1016/0005-2787(72)90115-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032678369
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1016/0005-2787(72)90217-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044275474
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1016/0005-2795(77)90052-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017454934
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1016/0304-4211(77)90116-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1028266837
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1016/0304-4211(78)90151-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019566278
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1016/s0015-3796(17)30861-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084042461
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1016/s0015-3796(17)30984-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084042551
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1073/pnas.74.12.5487 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004512076
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1073/pnas.74.3.1082 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002466251
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1073/pnas.74.8.3193 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030319913
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1093/oxfordjournals.jhered.a108685 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083485379
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1126/science.1108201 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062451473
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1126/science.187.4174.353 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062511016
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1126/science.190.4221.1304 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062512924
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1515/znc-1973-11-1215 schema:sameAs https://app.dimensions.ai/details/publication/pub.1067583450
202 rdf:type schema:CreativeWork
203 https://www.grid.ac/institutes/grid.418674.8 schema:alternateName Carlsberg Laboratory
204 schema:name Department of Physiology, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Copenhagen, Valby
205 rdf:type schema:Organization
206 https://www.grid.ac/institutes/grid.419580.1 schema:alternateName Max Planck Institute for Biology
207 schema:name Max-Planck-Institut für Biologie, Corrensstrasse 45, D-7400, Tübingen
208 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...