The barley chloroplast genome: Physical structure and transcriptional activity in vivo View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1983-01

AUTHORS

Carsten Poulsen

ABSTRACT

DNA has been isolated from barley chloroplasts and analyzed with restriction endonucleases PstI, SalI, PvuII and HindIII. Fragments obtained by digestion with PstI and HindIII have been inserted in the bacterial transformation vectors pBR325 and pBR322 and amplified in Escherichia coli. The inserts of the recombinant plasmids have been mapped with the same restriction enzymes as the intact chloroplast DNA yielding a physical map for the barley chloroplast genome. It is a circular molecule about 133,000 basepairs in size, which is equivalent to the chloroplast genomes of other gramineae such as wheat and maize. Like these it has an inverted repeat, of about 20,900 basepairs, containing the genes for the ribosomal RNAs. There are many similarities of the barley, wheat and maize genomes with respect to recognition sites for the enzymes PstI, SalI and PvuII. Heterologous hybridization with a probe containing parts of the genes for the large subunit of ribulose bisphosphate carboxylase and for the dicistronic mRNA for two ATPsynthetase CF; subunits, reveal that the position and organization of these genes in the barley chloroplast DNA are the same as found for maize and wheat. Selected chloroplast DNA fragments isolated from the recombinant plasmids and covering about 80% of the genome have been used for hybridization to RNA purified from plastids of dark grown and 8 hours illuminated seedlings. The RNA was electrophoretically separated in denaturing agarose gels and subsequently Northern blotted onto DBM-paper. The DNA fragments were nick translated and hybridized to the filterbound RNA. A total of 70 transcripts ranging in size from 350 nucleotides to more than 6,000 were identified. Of these 11 were synthesized only after illumination of the seedlings. The transcripts encoding the large subunit of ribulose bisphosphate carboxylase and the ATP synthetase CF1 subunits β and ε have been tentatively identified. About 30 transcripts are large than 3,000 nucleotides, indicating either the existence of a large number of polycistronic mRNAs or various forms of precursor RNAs. More... »

PAGES

57-80

References to SciGraph publications

Journal

TITLE

Carlsberg Research Communications

ISSUE

1

VOLUME

48

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02906170

DOI

http://dx.doi.org/10.1007/bf02906170

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1033461006


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Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf02906170'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf02906170'


 

This table displays all metadata directly associated to this object as RDF triples.

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