A new method for the detection of viable cells in tissue sections using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT): an application in the ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-12

AUTHORS

J. A. Radosevich, G. K. Haines, K. M. Elseth, G. E. Shambaugh, V. K. Maker

ABSTRACT

This report describes a new method using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to visualize live viable tissue at the microscopic level. The visualization of the MTT stained tissue provides a metabolic assessment of tissue damage, and can be utilized as an extension of conventional hematoxylin-eosin (H & E) staining. In this report, several tissues were studied with MTT and H & E staining after incisions had been made by a variety of surgical dissecting instruments. A marked improvement in the detection of tissue damage was seen using MTT, regardless of how the damage was caused, i.e., physical, heat, or photon energy. In addition, a distinct zone of damage not noted on conventionally prepared and stained tissues is readily apparent. Thus MTT staining will have utility in both clinical and research studies, concerned with assessing the viability of tissues. More... »

PAGES

345

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02899282

DOI

http://dx.doi.org/10.1007/bf02899282

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020163660

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7686698


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