The effect ofin vitro andin vivo ethylenbis dithiocarbamate fungicides on NMDA receptors in rat brain membranes View Full Text


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Article Info

DATE

2001-04

AUTHORS

Nobuhiro Konno, Masashi Tsunoda, Kyoko Nakano

ABSTRACT

To determine whether the ethlenbisdithiocarbamate fungicides, zineb, manzeb and maneb affect the N-methyl-D-aspartate (NMDA) receptor in rat brain membranes, we performed a binding assay using [3H]MK-801, a noncompetitive NMDA receptor antagonist. Displacement studies were conducted using well washed membranes to exclude the effect of endogenous acidic amino acids on the binding of [3H]MK-801. In both the presence or absence of added glutamate and glycine in the assay buffer, the dose-response curve indicated that zineb enhanced the binding in a concentration range of 100–500 μM. However, the displacement curves indicated that manzeb and maneb inhibited the binding in a concentration range of 10–500 μM. The addition of 50 μM glutamate and glycine to the assay medium increased binding by 5–20% above the control in a concentration range of 0.1–100 μM.No rats injected with zineb, manzeb, maneb (100 mg/kg, ip) showed any characteristic toxic signs or any significant weight changes within 24 hrs. Estimation of [3H]MK-801 binding to unwashed membranes from intoxicated rat brains revealed no marked change in Bmax or Kd values for 24 hrs following fungicide administration. More... »

PAGES

54-59

References to SciGraph publications

  • 1983. Acute Central Nervous System Changes due to Intoxication by Manzidan (A Combined Dithiocarbamate of Maneb and Zineb) in TOXICOLOGY IN THE USE, MISUSE, AND ABUSE OF FOOD, DRUGS, AND CHEMICALS
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    http://scigraph.springernature.com/pub.10.1007/bf02897310

    DOI

    http://dx.doi.org/10.1007/bf02897310

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21432238


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