Construction of eukaryotic expressing plasmids encodingHA andHA1 of influenza A virus and their transient expression in HEK293 cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-03

AUTHORS

Zhang Weidong, Li Mingyuan, Cao Kang, Yang Jing, Shi Qiaofa, Wang Baoning, Jiang Zhonghua, Li Hong

ABSTRACT

In order to explore the feasibility and protective efficiency of influenza DNA vaccine, we constructed eukaryotic expressing plasmids encoding HA and HA1 of influenza A virus (A/PR/8/34) and studied their expression in HEK293 cells. HA and HA1 genes were amplified by RT-PCR and cloned into pcDNA3.1(+) to generate pcDNA3.1(+)/HA and pcDNA3.1(+)/HA1, respectively. After verification of the cloning fidelity by restriction endonuclease digestion, PCR, and sequencing, pcDNA3.1(+)/HA and pcDNA3.1(+)/HA1 were transfected into HEK293 cells using PolyFect Transfection Reagent. Immunofluorescence assay was used to detect the transient expressing cells. Fluorescence microscopy revealed strong expression of target gene in HEK293 cells transiently transfected with either pcDNA3.1(+)/HA or pcDNA3.1(+)/HA1. Therefore, the results confirm the successful construction of eukaryotic expressing plasmids capable of driving the eukaryotic expression of influenza virus antigen HA and HA1, which is likely to provide a basis for both further investigation of the mechanism of influenza viral infection and the development of influenza DNA vaccine. More... »

PAGES

225-227

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02895822

DOI

http://dx.doi.org/10.1007/bf02895822

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029195631

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16850753


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Eukaryotic Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorescent Antibody Technique", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hemagglutinin Glycoproteins, Influenza Virus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Influenza A virus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Influenza Vaccines", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Fluorescence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Fusion Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reverse Transcriptase Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transfection", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Vaccines, DNA", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Hebei Medical University", 
          "id": "https://www.grid.ac/institutes/grid.256883.2", 
          "name": [
            "Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, China", 
            "Department of Pathobiology, School of Basic Medicine of Hebei Medical University, 050017, Shijiazhuang, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Weidong", 
        "givenName": "Zhang", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Sichuan University", 
          "id": "https://www.grid.ac/institutes/grid.13291.38", 
          "name": [
            "Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mingyuan", 
        "givenName": "Li", 
        "id": "sg:person.0612713753.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0612713753.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Sichuan University", 
          "id": "https://www.grid.ac/institutes/grid.13291.38", 
          "name": [
            "Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kang", 
        "givenName": "Cao", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Sichuan University", 
          "id": "https://www.grid.ac/institutes/grid.13291.38", 
          "name": [
            "Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jing", 
        "givenName": "Yang", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Sichuan University", 
          "id": "https://www.grid.ac/institutes/grid.13291.38", 
          "name": [
            "Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Qiaofa", 
        "givenName": "Shi", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Sichuan University", 
          "id": "https://www.grid.ac/institutes/grid.13291.38", 
          "name": [
            "Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Baoning", 
        "givenName": "Wang", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Sichuan University", 
          "id": "https://www.grid.ac/institutes/grid.13291.38", 
          "name": [
            "Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhonghua", 
        "givenName": "Jiang", 
        "id": "sg:person.01043370753.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01043370753.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Sichuan University", 
          "id": "https://www.grid.ac/institutes/grid.13291.38", 
          "name": [
            "Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hong", 
        "givenName": "Li", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1146/annurev.immunol.15.1.617", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000338683"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0167-5699(97)01201-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004490790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vaccine.2003.10.045", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014455392"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0264-410x(93)90385-b", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023595172"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0264-410x(93)90385-b", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023595172"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/292072a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044533628", 
          "https://doi.org/10.1038/292072a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-03", 
    "datePublishedReg": "2006-03-01", 
    "description": "In order to explore the feasibility and protective efficiency of influenza DNA vaccine, we constructed eukaryotic expressing plasmids encoding HA and HA1 of influenza A virus (A/PR/8/34) and studied their expression in HEK293 cells. HA and HA1 genes were amplified by RT-PCR and cloned into pcDNA3.1(+) to generate pcDNA3.1(+)/HA and pcDNA3.1(+)/HA1, respectively. After verification of the cloning fidelity by restriction endonuclease digestion, PCR, and sequencing, pcDNA3.1(+)/HA and pcDNA3.1(+)/HA1 were transfected into HEK293 cells using PolyFect Transfection Reagent. Immunofluorescence assay was used to detect the transient expressing cells. Fluorescence microscopy revealed strong expression of target gene in HEK293 cells transiently transfected with either pcDNA3.1(+)/HA or pcDNA3.1(+)/HA1. Therefore, the results confirm the successful construction of eukaryotic expressing plasmids capable of driving the eukaryotic expression of influenza virus antigen HA and HA1, which is likely to provide a basis for both further investigation of the mechanism of influenza viral infection and the development of influenza DNA vaccine.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf02895822", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1032019", 
        "issn": [
          "2096-5230", 
          "2523-899X"
        ], 
        "name": "ACTA ACADEMIAE MEDICINAE WUHAN", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "26"
      }
    ], 
    "name": "Construction of eukaryotic expressing plasmids encodingHA andHA1 of influenza A virus and their transient expression in HEK293 cells", 
    "pagination": "225-227", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "8b15b00beac4b887268ba67f889aaf324467ce115555a05312eb04fb1203f739"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "16850753"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101169627"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf02895822"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1029195631"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf02895822", 
      "https://app.dimensions.ai/details/publication/pub.1029195631"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:32", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000349_0000000349/records_113658_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF02895822"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf02895822'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf02895822'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf02895822'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf02895822'


 

This table displays all metadata directly associated to this object as RDF triples.

192 TRIPLES      21 PREDICATES      49 URIs      36 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf02895822 schema:about N06acccbab47f4a079f07e829b3ecc1ff
2 N08de09c8e4c44bf2b538ac3024b0c7df
3 N1057d13b74ea4de5a713ef63cbd989fa
4 N1ffb8e559af64da5b6a6ad26dd5c8334
5 N40ede379b5274e238bdf040863c16956
6 N45849033767a4575ba37c8d4eff6c2e3
7 N4c90d55b5ba24a15ba9cde9184207fa6
8 Nac4f3dd0c29b43a6b7759ba70ba5be0f
9 Ndf5c8e98e4344fbbbd93739923ebddf1
10 Ne147ad529c1441c6a20fec9581f16cf3
11 Ne28b1684db1544c08cb1c8323b7fbe35
12 Ne7ba043f34c8437a8b11e71cb8c7e073
13 Ne9383a66cba94ddcab3d14543f5ab391
14 Nebad829735a9448fb7f82283c475165d
15 Nf77986d715484051a2c88eeaec324daa
16 anzsrc-for:11
17 anzsrc-for:1107
18 schema:author Nd7aebd04d79e459ebc0a6854523e108a
19 schema:citation sg:pub.10.1038/292072a0
20 https://doi.org/10.1016/0264-410x(93)90385-b
21 https://doi.org/10.1016/j.vaccine.2003.10.045
22 https://doi.org/10.1016/s0167-5699(97)01201-2
23 https://doi.org/10.1146/annurev.immunol.15.1.617
24 schema:datePublished 2006-03
25 schema:datePublishedReg 2006-03-01
26 schema:description In order to explore the feasibility and protective efficiency of influenza DNA vaccine, we constructed eukaryotic expressing plasmids encoding HA and HA1 of influenza A virus (A/PR/8/34) and studied their expression in HEK293 cells. HA and HA1 genes were amplified by RT-PCR and cloned into pcDNA3.1(+) to generate pcDNA3.1(+)/HA and pcDNA3.1(+)/HA1, respectively. After verification of the cloning fidelity by restriction endonuclease digestion, PCR, and sequencing, pcDNA3.1(+)/HA and pcDNA3.1(+)/HA1 were transfected into HEK293 cells using PolyFect Transfection Reagent. Immunofluorescence assay was used to detect the transient expressing cells. Fluorescence microscopy revealed strong expression of target gene in HEK293 cells transiently transfected with either pcDNA3.1(+)/HA or pcDNA3.1(+)/HA1. Therefore, the results confirm the successful construction of eukaryotic expressing plasmids capable of driving the eukaryotic expression of influenza virus antigen HA and HA1, which is likely to provide a basis for both further investigation of the mechanism of influenza viral infection and the development of influenza DNA vaccine.
27 schema:genre research_article
28 schema:inLanguage en
29 schema:isAccessibleForFree false
30 schema:isPartOf N5e014a44a33a4047a8757f3b953bc777
31 Ne4da87b57d1b49afa7370a72db6371b3
32 sg:journal.1032019
33 schema:name Construction of eukaryotic expressing plasmids encodingHA andHA1 of influenza A virus and their transient expression in HEK293 cells
34 schema:pagination 225-227
35 schema:productId N14acc5c992d64fe0a7ff6b5b61bc752f
36 N19a623621aba4481ac2d34e133d6ba40
37 N279b419bf10d4440a034d2e7a6dbdfac
38 N5cf30cb2d50544419a50590980666406
39 Ncf26cd2747524da0afad30b9f28d0b8c
40 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029195631
41 https://doi.org/10.1007/bf02895822
42 schema:sdDatePublished 2019-04-11T10:32
43 schema:sdLicense https://scigraph.springernature.com/explorer/license/
44 schema:sdPublisher Nae7b0f7747fb4c719928806d7b2cb90f
45 schema:url http://link.springer.com/10.1007/BF02895822
46 sgo:license sg:explorer/license/
47 sgo:sdDataset articles
48 rdf:type schema:ScholarlyArticle
49 N057de5c8ced949abadc3588dba4e3e6d rdf:first Nc3f4dce1e47b46a0af6db32c31b01817
50 rdf:rest Nb864dc97094f49a0bb0a52ae446ba114
51 N06acccbab47f4a079f07e829b3ecc1ff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
52 schema:name Influenza Vaccines
53 rdf:type schema:DefinedTerm
54 N08de09c8e4c44bf2b538ac3024b0c7df schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
55 schema:name Eukaryotic Cells
56 rdf:type schema:DefinedTerm
57 N0d1c131762ca482e99020821a9ac199e rdf:first N417abe81d6e246ba89f472330cf8e314
58 rdf:rest N057de5c8ced949abadc3588dba4e3e6d
59 N1057d13b74ea4de5a713ef63cbd989fa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
60 schema:name Plasmids
61 rdf:type schema:DefinedTerm
62 N14acc5c992d64fe0a7ff6b5b61bc752f schema:name doi
63 schema:value 10.1007/bf02895822
64 rdf:type schema:PropertyValue
65 N19a623621aba4481ac2d34e133d6ba40 schema:name dimensions_id
66 schema:value pub.1029195631
67 rdf:type schema:PropertyValue
68 N1ffb8e559af64da5b6a6ad26dd5c8334 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name Microscopy, Fluorescence
70 rdf:type schema:DefinedTerm
71 N279b419bf10d4440a034d2e7a6dbdfac schema:name pubmed_id
72 schema:value 16850753
73 rdf:type schema:PropertyValue
74 N2ecd92ea08ab4eda8388f97f68817a43 schema:affiliation https://www.grid.ac/institutes/grid.13291.38
75 schema:familyName Hong
76 schema:givenName Li
77 rdf:type schema:Person
78 N3a5e8391b5db4c1286713ec92a33e803 rdf:first sg:person.01043370753.22
79 rdf:rest Nc132985d203a4789945cf13a4090a27b
80 N40ede379b5274e238bdf040863c16956 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Fluorescent Antibody Technique
82 rdf:type schema:DefinedTerm
83 N417abe81d6e246ba89f472330cf8e314 schema:affiliation https://www.grid.ac/institutes/grid.13291.38
84 schema:familyName Kang
85 schema:givenName Cao
86 rdf:type schema:Person
87 N4384fc54872044d8aad337641fa4095c rdf:first sg:person.0612713753.26
88 rdf:rest N0d1c131762ca482e99020821a9ac199e
89 N45849033767a4575ba37c8d4eff6c2e3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Influenza A virus
91 rdf:type schema:DefinedTerm
92 N4c90d55b5ba24a15ba9cde9184207fa6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Reverse Transcriptase Polymerase Chain Reaction
94 rdf:type schema:DefinedTerm
95 N5cf30cb2d50544419a50590980666406 schema:name nlm_unique_id
96 schema:value 101169627
97 rdf:type schema:PropertyValue
98 N5e014a44a33a4047a8757f3b953bc777 schema:volumeNumber 26
99 rdf:type schema:PublicationVolume
100 N724491e0a0ba495383ec22af2efdd404 schema:affiliation https://www.grid.ac/institutes/grid.256883.2
101 schema:familyName Weidong
102 schema:givenName Zhang
103 rdf:type schema:Person
104 N72e76b9f5c7c43dc8e3ed9ba1ac26685 schema:affiliation https://www.grid.ac/institutes/grid.13291.38
105 schema:familyName Qiaofa
106 schema:givenName Shi
107 rdf:type schema:Person
108 Nac4f3dd0c29b43a6b7759ba70ba5be0f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Vaccines, DNA
110 rdf:type schema:DefinedTerm
111 Nae7b0f7747fb4c719928806d7b2cb90f schema:name Springer Nature - SN SciGraph project
112 rdf:type schema:Organization
113 Naf6536f494384d36bd168d54c3ad77a6 rdf:first Nedce7c7a5d61422ead18c3f8b3375a6a
114 rdf:rest N3a5e8391b5db4c1286713ec92a33e803
115 Nb864dc97094f49a0bb0a52ae446ba114 rdf:first N72e76b9f5c7c43dc8e3ed9ba1ac26685
116 rdf:rest Naf6536f494384d36bd168d54c3ad77a6
117 Nc132985d203a4789945cf13a4090a27b rdf:first N2ecd92ea08ab4eda8388f97f68817a43
118 rdf:rest rdf:nil
119 Nc3f4dce1e47b46a0af6db32c31b01817 schema:affiliation https://www.grid.ac/institutes/grid.13291.38
120 schema:familyName Jing
121 schema:givenName Yang
122 rdf:type schema:Person
123 Ncf26cd2747524da0afad30b9f28d0b8c schema:name readcube_id
124 schema:value 8b15b00beac4b887268ba67f889aaf324467ce115555a05312eb04fb1203f739
125 rdf:type schema:PropertyValue
126 Nd7aebd04d79e459ebc0a6854523e108a rdf:first N724491e0a0ba495383ec22af2efdd404
127 rdf:rest N4384fc54872044d8aad337641fa4095c
128 Ndf5c8e98e4344fbbbd93739923ebddf1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Transfection
130 rdf:type schema:DefinedTerm
131 Ne147ad529c1441c6a20fec9581f16cf3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Humans
133 rdf:type schema:DefinedTerm
134 Ne28b1684db1544c08cb1c8323b7fbe35 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Cell Line
136 rdf:type schema:DefinedTerm
137 Ne4da87b57d1b49afa7370a72db6371b3 schema:issueNumber 2
138 rdf:type schema:PublicationIssue
139 Ne7ba043f34c8437a8b11e71cb8c7e073 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Gene Expression
141 rdf:type schema:DefinedTerm
142 Ne9383a66cba94ddcab3d14543f5ab391 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Hemagglutinin Glycoproteins, Influenza Virus
144 rdf:type schema:DefinedTerm
145 Nebad829735a9448fb7f82283c475165d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Cloning, Molecular
147 rdf:type schema:DefinedTerm
148 Nedce7c7a5d61422ead18c3f8b3375a6a schema:affiliation https://www.grid.ac/institutes/grid.13291.38
149 schema:familyName Baoning
150 schema:givenName Wang
151 rdf:type schema:Person
152 Nf77986d715484051a2c88eeaec324daa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Recombinant Fusion Proteins
154 rdf:type schema:DefinedTerm
155 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
156 schema:name Medical and Health Sciences
157 rdf:type schema:DefinedTerm
158 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
159 schema:name Immunology
160 rdf:type schema:DefinedTerm
161 sg:journal.1032019 schema:issn 2096-5230
162 2523-899X
163 schema:name ACTA ACADEMIAE MEDICINAE WUHAN
164 rdf:type schema:Periodical
165 sg:person.01043370753.22 schema:affiliation https://www.grid.ac/institutes/grid.13291.38
166 schema:familyName Zhonghua
167 schema:givenName Jiang
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01043370753.22
169 rdf:type schema:Person
170 sg:person.0612713753.26 schema:affiliation https://www.grid.ac/institutes/grid.13291.38
171 schema:familyName Mingyuan
172 schema:givenName Li
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0612713753.26
174 rdf:type schema:Person
175 sg:pub.10.1038/292072a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044533628
176 https://doi.org/10.1038/292072a0
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1016/0264-410x(93)90385-b schema:sameAs https://app.dimensions.ai/details/publication/pub.1023595172
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1016/j.vaccine.2003.10.045 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014455392
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1016/s0167-5699(97)01201-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004490790
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1146/annurev.immunol.15.1.617 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000338683
185 rdf:type schema:CreativeWork
186 https://www.grid.ac/institutes/grid.13291.38 schema:alternateName Sichuan University
187 schema:name Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, China
188 rdf:type schema:Organization
189 https://www.grid.ac/institutes/grid.256883.2 schema:alternateName Hebei Medical University
190 schema:name Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, China
191 Department of Pathobiology, School of Basic Medicine of Hebei Medical University, 050017, Shijiazhuang, China
192 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...