The effect of pyruvic acid thiosemicarbazone on ornithine carbamoyl transferase ofLycopersicon esculentumMill View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-05

AUTHORS

J. Miersch, G. -J. Krauss, K. Grancharov, F. Bublitz, N. Spassovska, E. Golovinsky

ABSTRACT

The pyruvic acid thiosemicarbazone was found to inhibitin vitro the soluble ornithine carbamoyl transferase as well as the enzyme in a mitochondria-rich cell fraction. For proving the inhibition of ornithine carbamoyl transferase in mitochondria-rich fraction a new method for DEAE-thin-layer chromatography of radiocarbon labelled citrulline and ornithine was used. The mitochondria prepared by discontinuous sucrose density gradient centrifugation are physiologically active and oxidized succinate under state 3 conditions. The respiratory control ratio was determined to 3.3. The results obtained show that this analogue of pyruvic acid is an inhibitor of ornithine carbamoyl transferase. More... »

PAGES

174-179

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02894593

DOI

http://dx.doi.org/10.1007/bf02894593

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1051957865


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