Regulation of the formation of protease inBacillus megaterium View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1966-03

AUTHORS

J. Chaloupka, Pavla Křečková

ABSTRACT

Protease is synthesized by the cultures growing in a glucose-containing mineral medium. However, it is formed even during incubation of the washed cells in a nitrogen free medium. The enzyme synthesis is decreased substantially by the addition of the individual amino acids or their mixture. Threonine, isoleucine, leucine and valine are the most inhibitory. Arginine, cysteine, glycine, lysine and tryptophan in concentrations of 103m do not inhibit the production of protease. The growth of the culture is also somewhat inhibited by threonine and isoleucine, the repression of protease being, however, much higher. Concentrations of 103m inhibit its synthesis by 80–90%. However, the enzyme activity is not influenced. The inhibition is caused byl,-isomers. Repression of the enzyme synthesis after the addition of threonine into the medium is much greater in a growing culture than in a culture starving in a nitrogen-free medium. However the level of free threonine in the pool is roughly the same in both growing and non-growing cultures. A mixture of 13 amino acids, which themselves are little inhibitory, suppresses the synthesis of protease much more than threonine or isoleucine. The inhibitory effect of the individual amino acids on the enzyme formation is apparently additive. More... »

PAGES

82-88

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02878835

DOI

http://dx.doi.org/10.1007/bf02878835

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1039964484

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/4958280


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