Fractionation of a population ofSaccharomyces cerevisiae yeasts by centrifugation in a dextran gradient View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1964-07

AUTHORS

Jitka Lieblová, K. Beran, Eva Streiblová

ABSTRACT

A method of the fractionation of aSaccharomyces cerevisiae yeast population in dextran gradients is described. The elaboration of this method was based on the finding of a correlation between the size of individual cells and the number of bud scars on their surface and rapid indication of the scars by fluorescence microscopy. The basic conditions for fractionation (determined experimentally) were as follows: 2 ml. yeast suspension (100 mg. dry weight) was applied to the surface of a continuous dextran gradient of 9–16% concentration and was centrifuged at a relative centrifugal force of 200 G for 15 minutes. In fractionation of a whole population, the best fractionation was obtained in a linear gradient. Repeated separation of fractions obtained by centrifugation in a linear gradient in a concave gradient further separated cells without bud scars and accumulated cells with five scars and over. Three fractions were obtained by this technique. The first contained 90–98% cells without bud scars, the second 55–65% cells with 1–4 bud scars and the third 50% cells with five bud scars and over. More... »

PAGES

205-213

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02875838

DOI

http://dx.doi.org/10.1007/bf02875838

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036274566

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/14190690


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