Potato tissue cultures View Full Text


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Article Info

DATE

1955-06

AUTHORS

H. W. Chapman

ABSTRACT

Nodes excised from potato sprouts and rendered aseptic with hypochlorite developed to morphologically complete plants in one ounce bottles. White’s medium with 0.7 per cent agar added was an excellent growth medium. Undifferentiated masses of tissue grew readily on the basal medium plus 10 per cent coconut milk and 7mg/1 2,4-D. Root tips remained alive for some time but did not elongate when supplied with any of several different media. Tuberization was most rapid when culture were held in the light for four weeks at room temperature followed by several days in darkness at 60°F. Varying the N level of the medium exhibited no effect on rate of tuberization. Nodes grew eqally well with glucose, sucrose, or fructose as a carbohydrate supply. From 2 to 4 per cent sugar in the media appeared to be near the optimum concentration. More... »

PAGES

207-210

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02867788

DOI

http://dx.doi.org/10.1007/bf02867788

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1003264470


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