A rapid micro-starch quantitation method for potato callus and its application with potato tubers View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1974-12

AUTHORS

J. L. Varns, J. R. Sowokinos

ABSTRACT

The procedure described was developed as a micro-starch method for potato callus tissue (0.1-0.2% starch on a fresh weight basis) and then evaluated with macro-starch samples of potato tubers harvested during three physiological stages of development. This method consisted of the following steps: (a) a mild alkaline-sonic extraction of starch from lyophilized 80% aqueous ethanol-washed samples, (b) starch hydrolysis of the neutralized, filtered extracts by 1 N HC1, and (c) the determination of liberated glucose by the specific glucose oxidase system. Results with this procedure were as sensitive and accurate (SD ± 0.86%) as with other starch-specific methods with more extensive starch isolation and purification steps. More... »

PAGES

383-392

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02852113

DOI

http://dx.doi.org/10.1007/bf02852113

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049920803


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