Cross-amplification of microsatellite loci in a neotropicalQuercus species and standardization of DNA extraction from mature leaves dried in silica gel View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-12

AUTHORS

Juan F. Fern’andez, Victoria L. Sork, Gerardo Gallego, Jershon L’opez, Adriana Bohorques, Joe Tohme

ABSTRACT

Conservation of 15 out of 24 previously identified microsatellite loci (62.5%), was found in a survey of the South American oak,Quercus humboldtii. The number of alleles per locus varied from 2 to 20, detecting at least 5 microsatellite loci with 5 or more alleles. This number of loci and alleles is adequate for most studies of genetic diversity and gene flow analysis. In addition, a method for extracting DNA from mature oak leaves is described that minimizes oxidation of tannins, a common problem in silica-gel-dried samples. The microsatellite markers detected in this study and the DNA extraction protocol may be applied to more than 30 species ofQuercus that exist in tropical America. More... »

PAGES

397-397

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02825070

DOI

http://dx.doi.org/10.1007/bf02825070

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030994610


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