Stable integration and expression of β-glucuronidase and NPT II genes in mango somatic embryos View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-10

AUTHORS

Helena Mathews, R. E. Litz, H. D. Wilde, S. A. Merkle, H. Y. Wetzstein

ABSTRACT

Somatic proembryos of mango (Mangifera indica L. cv. Hindi) were co-cultivated withAgrobacterium tumefaciens strain A208 harboring pTiT37-Se::pMON 9749 (9749 ASE). Transformed somatic proembryos capable of growing on selection medium containing 200 μg/ml kanamycin produced the characteristic indigo blue precipitate in the presence of 5-bromo-4-chloro-3-glucuronic acid. These proembryos were chimeral consisting of transformed (blue) and nontransformed (yellow/white) cells. A stepwise selection strategy was found necessary to eliminate chimeras. a) Initial screening at 200 μg/ml kanamycin to enable growth of transformed cells, b) further screening at 400 μg/ml kanamycin to reduce chimeras, and c) recovery of pure transformed clones of proembryos in liquid selection medium with 100 μg/ml kanamycin. The integration of the NPT II and GUS genes into mango genome was confirmed by Southern hybridization. More... »

PAGES

172-178

References to SciGraph publications

  • 1990-08. Stable transformation of papaya via microprojectile bombardment in PLANT CELL REPORTS
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  • 1989-03. Genetic transformation of apple (Malus pumila Mill.) using a disarmed Ti-binary vector in PLANT CELL REPORTS
  • 1982-12. Somatic embryos from culture ovules of polyembryonic Mangifera indica L. in PLANT CELL REPORTS
  • 1990-01. Improved efficiency of the walnut somatic embryo gene transfer system in PLANT CELL REPORTS
  • 1985-03. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues in PLANT MOLECULAR BIOLOGY
  • 1987-12. Assaying chimeric genes in plants: The GUS gene fusion system in PLANT MOLECULAR BIOLOGY REPORTER
  • 1985-07. The SEV System: A New Disarmed Ti Plasmid Vector System for Plant Transformation in NATURE BIOTECHNOLOGY
  • 1990-11. Agrobacterium-Mediated Genetic Transformation of Grapevines: Transgenic Plants of Vitis rupestris SCHEELE and Buds of Vitis vinifera L. in NATURE BIOTECHNOLOGY
  • 1987-02. Agrobacterium mediated transformation and regeneration of Populus in MOLECULAR GENETICS AND GENOMICS
  • 1988-08. Stable Transformation of Soybean (Glycine Max) by Particle Acceleration in NATURE BIOTECHNOLOGY
  • 1988-07. Agrobacterium-Mediated Transformation of Walnut Somatic Embryos and Regeneration of Transgenic Plants in NATURE BIOTECHNOLOGY
  • 1991-02. Stable transformation of Populus and incorporation of pest resistance by electric discharge particle acceleration in PLANT CELL REPORTS
  • 1990-01. Use of the GUS gene as a selectable marker for Agrobacterium-mediated transformation of Rubus in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02823312

    DOI

    http://dx.doi.org/10.1007/bf02823312

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1033827028


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