PEG-mediated plastid transformation in higher plants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-10

AUTHORS

Waltraud Kofer, Christian Eibl, Klaus Steinmüller, Hans-Ulrich Koop

ABSTRACT

Plastids are surrounded by an envelope consisting of a double membrane. This barrier has to be penetrated or overcome by the DNA when transforming the plastome. Both the biolistic and polyethylene glycol-mediated transformation techniques accomplish this task, albeit by different mechanisms. We were the first laboratory to successfully use the polyethylene glycol (PEG)-method for plastid transformation, yet we use the particle gun when appropriate. In this report we compare the two methods and discuss their shortcomings and advantages. Plastid transformations with various constructs, mainly using theaadA gene as a selective marker, were performed. We point to potential problems likely to be encountered during the transformation and selection processes and offer possibilities for improvement. We give further examples of the successful application of plastome transformation and show its merits in addressing biological questions concerning the elucidation of plastid sequences of unknown function and the control of plastid gene expression. More... »

PAGES

303-309

Journal

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02822739

    DOI

    http://dx.doi.org/10.1007/bf02822739

    DIMENSIONS

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