In vitro induction of multiple shoots and plant regeneration inVigna View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-10

AUTHORS

Jayanti Sen, Sipra Guha-Mukherjee

ABSTRACT

Cotyledonary nodes, excised cotyledons, and hypocotyl segments of six varieties ofVigna mungo andV. radiata have been tested for their morphogenic potential on media containing a range of hormonal combinations including benzyladenine, kinetin, thidiazuron (TDZ), and zeatin. Multiple shoots developed on cotyledonary node explants in all varieties tested on basal medium containing cytokinin. Presence of both the cotyledons, either full or half, resulted in a maximum number of shoots produced. Shoot bud regeneration was achieved via meristem formation on excised cotyledons on Murashige-skoog basal medium with B5 vitamins supplemented with TDZ. Mature plants had normal phenotypes.V. mungo var. PS1 andV. radiata var. Pusa 105 were found to be the most responsive varieties for shoot regneration. The histology ofin vitro organogenesis was studied. More... »

PAGES

276-280

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02822733

DOI

http://dx.doi.org/10.1007/bf02822733

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021467725


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