Cytotoxic T lymphocyte activity to hepatitis B virus DNA-transfected HepG2 cells in patients with chronic hepatitis B View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-10

AUTHORS

Yuji Ito, Shinichi Kakumu, Kentaro Yoshioka, Takaji Wakita, Tetsuya Ishikawa, Katsuro Koike

ABSTRACT

Cytotoxic T lymphocyte (CTL) activity for hepatitis B virus (HBV) DNA-transfected HepG2 cells (designated HB3-5), which secrete HBsAg, HBeAg and HBV particles, was investigated in 31 patients with chronic HBV infection (18 chronic hepatitis and 13 asymptomatic carriers). 51Cr-labeled HB3-5 with A2 as a major HLA class I antigen served as target cells and T cells from peripheral blood mononuclear cells as effector cells. The CTL activity was measured by a 51Cr release assay. Patients were divided into two groups, the A2 group bearing HLA-A2 and the non-A2 group not bearing HLA-A2. Chronic hepatitis patients in the A2 group showed increased HBV Ag-specific cytotoxicity compared with that seen in the non-A2 group (5.2 +/- 3.1% vs. 0.9 +/- 1.4%; means +/- SD, P < 0.01). In the A2 group with chronic hepatitis, the cytotoxicity was greater in anti-HBe positive patients than in HBeAg positive patients (8.6 +/- 1.9% vs. 3.4 +/- 2.0%, P < 0.01), and asymptomatic carriers showed less cytotoxicity (0.35 +/- 0.31%, P < 0.001) compared with chronic hepatitis patients. In the non-A2 group, HBV Ag-specific CTL activity was negligible in most patients and thus no differences were found among all patient groups. The HBV Ag-specific cytotoxicity was inhibited by antibodies to CD3, HLA class I and hepatitis B nucleocapsid antigens. Removal of CD8+ cells also resulted in marked decrease in the cytotoxicity. These findings indicate that HBV Ag-specific cytotoxicity reflects liver cell damage and HBeAg/anti-HBe status. Furthermore, our assay system appears to be useful to assess CTL response in patients with chronic HBV infection. More... »

PAGES

657-665

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02806346

DOI

http://dx.doi.org/10.1007/bf02806346

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1082468273

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7693538


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adult", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytotoxicity, Immunologic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epitopes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HLA-A2 Antigen", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hepatitis B", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hepatitis B Antibodies", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hepatitis B Antigens", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hepatitis B virus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hepatitis, Chronic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hepatoblastoma", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Liver Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Lymphocytes, Cytotoxic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transfection", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tumor Cells, Cultured", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Nagoya University", 
          "id": "https://www.grid.ac/institutes/grid.27476.30", 
          "name": [
            "Third Department of Internal Medicine, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, 466, Nagoya, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ito", 
        "givenName": "Yuji", 
        "id": "sg:person.015611624262.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015611624262.76"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nagoya University", 
          "id": "https://www.grid.ac/institutes/grid.27476.30", 
          "name": [
            "Third Department of Internal Medicine, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, 466, Nagoya, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kakumu", 
        "givenName": "Shinichi", 
        "id": "sg:person.0674115210.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0674115210.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nagoya University", 
          "id": "https://www.grid.ac/institutes/grid.27476.30", 
          "name": [
            "Third Department of Internal Medicine, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, 466, Nagoya, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yoshioka", 
        "givenName": "Kentaro", 
        "id": "sg:person.01276574006.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276574006.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nagoya University", 
          "id": "https://www.grid.ac/institutes/grid.27476.30", 
          "name": [
            "Third Department of Internal Medicine, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, 466, Nagoya, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wakita", 
        "givenName": "Takaji", 
        "id": "sg:person.0671521055.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0671521055.46"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nagoya University", 
          "id": "https://www.grid.ac/institutes/grid.27476.30", 
          "name": [
            "Third Department of Internal Medicine, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, 466, Nagoya, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ishikawa", 
        "givenName": "Tetsuya", 
        "id": "sg:person.01040603424.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01040603424.38"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Cancer Institute Hospital", 
          "id": "https://www.grid.ac/institutes/grid.486756.e", 
          "name": [
            "Department of Gene Research, Cancer Institute, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Koike", 
        "givenName": "Katsuro", 
        "id": "sg:person.012233615332.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012233615332.34"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/s0168-8278(87)80004-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002031509"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(88)91043-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002057631"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1159/000200935", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006432022"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(72)90234-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008474289"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4612-4850-7_13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009137583", 
          "https://doi.org/10.1007/978-1-4612-4850-7_13"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(83)90517-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012028100"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.84.9.2678", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015380064"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(73)90341-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017876073"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.84.4.1005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018363746"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.88.23.10445", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023861433"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.158.2.303", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028788686"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(86)90364-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029596455"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.174.6.1565", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030707982"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(74)90287-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034544658"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/204998b0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038198783", 
          "https://doi.org/10.1038/204998b0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0167-5699(88)91317-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043563368"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0167-5699(88)91317-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043563368"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-0676.1991.tb00500.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046809904"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-0676.1991.tb00500.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046809904"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1691527", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062500821"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.6248960", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062635849"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075488857", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078890904", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078936199", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079785412", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081652759", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081664770", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081728485", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081751737", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1993-10", 
    "datePublishedReg": "1993-10-01", 
    "description": "Cytotoxic T lymphocyte (CTL) activity for hepatitis B virus (HBV) DNA-transfected HepG2 cells (designated HB3-5), which secrete HBsAg, HBeAg and HBV particles, was investigated in 31 patients with chronic HBV infection (18 chronic hepatitis and 13 asymptomatic carriers). 51Cr-labeled HB3-5 with A2 as a major HLA class I antigen served as target cells and T cells from peripheral blood mononuclear cells as effector cells. The CTL activity was measured by a 51Cr release assay. Patients were divided into two groups, the A2 group bearing HLA-A2 and the non-A2 group not bearing HLA-A2. Chronic hepatitis patients in the A2 group showed increased HBV Ag-specific cytotoxicity compared with that seen in the non-A2 group (5.2 +/- 3.1% vs. 0.9 +/- 1.4%; means +/- SD, P < 0.01). In the A2 group with chronic hepatitis, the cytotoxicity was greater in anti-HBe positive patients than in HBeAg positive patients (8.6 +/- 1.9% vs. 3.4 +/- 2.0%, P < 0.01), and asymptomatic carriers showed less cytotoxicity (0.35 +/- 0.31%, P < 0.001) compared with chronic hepatitis patients. In the non-A2 group, HBV Ag-specific CTL activity was negligible in most patients and thus no differences were found among all patient groups. The HBV Ag-specific cytotoxicity was inhibited by antibodies to CD3, HLA class I and hepatitis B nucleocapsid antigens. Removal of CD8+ cells also resulted in marked decrease in the cytotoxicity. These findings indicate that HBV Ag-specific cytotoxicity reflects liver cell damage and HBeAg/anti-HBe status. Furthermore, our assay system appears to be useful to assess CTL response in patients with chronic HBV infection.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf02806346", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1009747", 
        "issn": [
          "0944-1174", 
          "1435-5922"
        ], 
        "name": "Journal of Gastroenterology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "28"
      }
    ], 
    "name": "Cytotoxic T lymphocyte activity to hepatitis B virus DNA-transfected HepG2 cells in patients with chronic hepatitis B", 
    "pagination": "657-665", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "b676957046d2a3c6b27f2689e1178a6b08d92eb8d19c050d7d33d32ce8c8a208"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "7693538"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0152744"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf02806346"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1082468273"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf02806346", 
      "https://app.dimensions.ai/details/publication/pub.1082468273"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T01:03", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8697_00000492.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF02806346"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf02806346'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf02806346'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf02806346'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf02806346'


 

This table displays all metadata directly associated to this object as RDF triples.

254 TRIPLES      21 PREDICATES      74 URIs      39 LITERALS      27 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf02806346 schema:about N29f27445c97b44e48d52482bfe59a98b
2 N6010dd61116f428bacdfe1d4d7a60a44
3 N63acbd51cc094d0ca42a747d05450d88
4 N66b5dac6442f47a99aa75814cc8426d4
5 N71f8a7e0825e48d38da5ae7521384e75
6 N78e629d5cb634bfd8179315cb3a139e7
7 N7a895bbe16fc477da023678277cb50c2
8 N8cbf493318a14e5f9f69fc67792b95c1
9 N8e6e98b17a934fcaaee1a142b66b4ed3
10 Nad1cc9a1e7b941e68fd72d1936153485
11 Naefd188fb6f548d0b3e7394ea4177a9c
12 Nba58aa94b0c8438ca65530a888ffe1f8
13 Nc0709356c2e0484192070eec1a3738e5
14 Nd37bf143214d4adcb9038529adefbb15
15 Nd5fec9b1eda44179bc664964135b37a6
16 Ndac643d438794cf485f5027a2192d1c3
17 Nf2002fefd0ca42c6a2621020014c74c0
18 Nf90ba665a4aa415388c0f4cce4aea5aa
19 anzsrc-for:11
20 anzsrc-for:1107
21 schema:author N14b6f5d4813b4bc482b4388c4912f506
22 schema:citation sg:pub.10.1007/978-1-4612-4850-7_13
23 sg:pub.10.1038/204998b0
24 https://app.dimensions.ai/details/publication/pub.1075488857
25 https://app.dimensions.ai/details/publication/pub.1078890904
26 https://app.dimensions.ai/details/publication/pub.1078936199
27 https://app.dimensions.ai/details/publication/pub.1079785412
28 https://app.dimensions.ai/details/publication/pub.1081652759
29 https://app.dimensions.ai/details/publication/pub.1081664770
30 https://app.dimensions.ai/details/publication/pub.1081728485
31 https://app.dimensions.ai/details/publication/pub.1081751737
32 https://doi.org/10.1016/0042-6822(73)90341-3
33 https://doi.org/10.1016/0092-8674(83)90517-2
34 https://doi.org/10.1016/0092-8674(86)90364-8
35 https://doi.org/10.1016/0167-5699(88)91317-5
36 https://doi.org/10.1016/s0092-8674(88)91043-4
37 https://doi.org/10.1016/s0140-6736(72)90234-6
38 https://doi.org/10.1016/s0140-6736(74)90287-6
39 https://doi.org/10.1016/s0168-8278(87)80004-1
40 https://doi.org/10.1073/pnas.84.4.1005
41 https://doi.org/10.1073/pnas.84.9.2678
42 https://doi.org/10.1073/pnas.88.23.10445
43 https://doi.org/10.1084/jem.158.2.303
44 https://doi.org/10.1084/jem.174.6.1565
45 https://doi.org/10.1111/j.1600-0676.1991.tb00500.x
46 https://doi.org/10.1126/science.1691527
47 https://doi.org/10.1126/science.6248960
48 https://doi.org/10.1159/000200935
49 schema:datePublished 1993-10
50 schema:datePublishedReg 1993-10-01
51 schema:description Cytotoxic T lymphocyte (CTL) activity for hepatitis B virus (HBV) DNA-transfected HepG2 cells (designated HB3-5), which secrete HBsAg, HBeAg and HBV particles, was investigated in 31 patients with chronic HBV infection (18 chronic hepatitis and 13 asymptomatic carriers). 51Cr-labeled HB3-5 with A2 as a major HLA class I antigen served as target cells and T cells from peripheral blood mononuclear cells as effector cells. The CTL activity was measured by a 51Cr release assay. Patients were divided into two groups, the A2 group bearing HLA-A2 and the non-A2 group not bearing HLA-A2. Chronic hepatitis patients in the A2 group showed increased HBV Ag-specific cytotoxicity compared with that seen in the non-A2 group (5.2 +/- 3.1% vs. 0.9 +/- 1.4%; means +/- SD, P < 0.01). In the A2 group with chronic hepatitis, the cytotoxicity was greater in anti-HBe positive patients than in HBeAg positive patients (8.6 +/- 1.9% vs. 3.4 +/- 2.0%, P < 0.01), and asymptomatic carriers showed less cytotoxicity (0.35 +/- 0.31%, P < 0.001) compared with chronic hepatitis patients. In the non-A2 group, HBV Ag-specific CTL activity was negligible in most patients and thus no differences were found among all patient groups. The HBV Ag-specific cytotoxicity was inhibited by antibodies to CD3, HLA class I and hepatitis B nucleocapsid antigens. Removal of CD8+ cells also resulted in marked decrease in the cytotoxicity. These findings indicate that HBV Ag-specific cytotoxicity reflects liver cell damage and HBeAg/anti-HBe status. Furthermore, our assay system appears to be useful to assess CTL response in patients with chronic HBV infection.
52 schema:genre research_article
53 schema:inLanguage en
54 schema:isAccessibleForFree false
55 schema:isPartOf Nace2daf2d4984420a9b53c15b6774194
56 Nd57e43e73a284dd68eb9b989939c169b
57 sg:journal.1009747
58 schema:name Cytotoxic T lymphocyte activity to hepatitis B virus DNA-transfected HepG2 cells in patients with chronic hepatitis B
59 schema:pagination 657-665
60 schema:productId N109e628ffa7d4c9681fdd775a7ccc425
61 N17abd125f1a2486bb329c56acefa0bfc
62 N25f1c7e1018241b3afccae3225ab7f0f
63 N28399e3cf752494990e06b5d1ef13447
64 N5023847f5f06431db03cae231873e781
65 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082468273
66 https://doi.org/10.1007/bf02806346
67 schema:sdDatePublished 2019-04-11T01:03
68 schema:sdLicense https://scigraph.springernature.com/explorer/license/
69 schema:sdPublisher N58b503faccb64c4c891f3cafebaec601
70 schema:url http://link.springer.com/10.1007/BF02806346
71 sgo:license sg:explorer/license/
72 sgo:sdDataset articles
73 rdf:type schema:ScholarlyArticle
74 N109e628ffa7d4c9681fdd775a7ccc425 schema:name doi
75 schema:value 10.1007/bf02806346
76 rdf:type schema:PropertyValue
77 N14b6f5d4813b4bc482b4388c4912f506 rdf:first sg:person.015611624262.76
78 rdf:rest N68e3a5c50a34464e872b4462dd9ac718
79 N17abd125f1a2486bb329c56acefa0bfc schema:name readcube_id
80 schema:value b676957046d2a3c6b27f2689e1178a6b08d92eb8d19c050d7d33d32ce8c8a208
81 rdf:type schema:PropertyValue
82 N25f1c7e1018241b3afccae3225ab7f0f schema:name dimensions_id
83 schema:value pub.1082468273
84 rdf:type schema:PropertyValue
85 N28399e3cf752494990e06b5d1ef13447 schema:name pubmed_id
86 schema:value 7693538
87 rdf:type schema:PropertyValue
88 N29f27445c97b44e48d52482bfe59a98b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Transfection
90 rdf:type schema:DefinedTerm
91 N3cd61b7dad0c4d95a3c29aefb120a02c rdf:first sg:person.0671521055.46
92 rdf:rest Nb286ab3345204346afc7ebe49cf0d75c
93 N5023847f5f06431db03cae231873e781 schema:name nlm_unique_id
94 schema:value 0152744
95 rdf:type schema:PropertyValue
96 N58b503faccb64c4c891f3cafebaec601 schema:name Springer Nature - SN SciGraph project
97 rdf:type schema:Organization
98 N6010dd61116f428bacdfe1d4d7a60a44 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Epitopes
100 rdf:type schema:DefinedTerm
101 N63acbd51cc094d0ca42a747d05450d88 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Adult
103 rdf:type schema:DefinedTerm
104 N66b5dac6442f47a99aa75814cc8426d4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name HLA-A2 Antigen
106 rdf:type schema:DefinedTerm
107 N68e3a5c50a34464e872b4462dd9ac718 rdf:first sg:person.0674115210.05
108 rdf:rest N8e2c816c222e4f75a52a02f465b3fe1c
109 N71f8a7e0825e48d38da5ae7521384e75 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Hepatitis B Antigens
111 rdf:type schema:DefinedTerm
112 N78e629d5cb634bfd8179315cb3a139e7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Female
114 rdf:type schema:DefinedTerm
115 N7a895bbe16fc477da023678277cb50c2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Humans
117 rdf:type schema:DefinedTerm
118 N8cbf493318a14e5f9f69fc67792b95c1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Hepatitis B
120 rdf:type schema:DefinedTerm
121 N8e2c816c222e4f75a52a02f465b3fe1c rdf:first sg:person.01276574006.35
122 rdf:rest N3cd61b7dad0c4d95a3c29aefb120a02c
123 N8e6e98b17a934fcaaee1a142b66b4ed3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Hepatitis, Chronic
125 rdf:type schema:DefinedTerm
126 Nace2daf2d4984420a9b53c15b6774194 schema:volumeNumber 28
127 rdf:type schema:PublicationVolume
128 Nad1cc9a1e7b941e68fd72d1936153485 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name DNA, Viral
130 rdf:type schema:DefinedTerm
131 Naefd188fb6f548d0b3e7394ea4177a9c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Hepatitis B virus
133 rdf:type schema:DefinedTerm
134 Nb286ab3345204346afc7ebe49cf0d75c rdf:first sg:person.01040603424.38
135 rdf:rest Nc42aedfe95e44c248612e9bf7904d116
136 Nba58aa94b0c8438ca65530a888ffe1f8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Hepatoblastoma
138 rdf:type schema:DefinedTerm
139 Nc0709356c2e0484192070eec1a3738e5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Liver Neoplasms
141 rdf:type schema:DefinedTerm
142 Nc42aedfe95e44c248612e9bf7904d116 rdf:first sg:person.012233615332.34
143 rdf:rest rdf:nil
144 Nd37bf143214d4adcb9038529adefbb15 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name T-Lymphocytes, Cytotoxic
146 rdf:type schema:DefinedTerm
147 Nd57e43e73a284dd68eb9b989939c169b schema:issueNumber 5
148 rdf:type schema:PublicationIssue
149 Nd5fec9b1eda44179bc664964135b37a6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Male
151 rdf:type schema:DefinedTerm
152 Ndac643d438794cf485f5027a2192d1c3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Tumor Cells, Cultured
154 rdf:type schema:DefinedTerm
155 Nf2002fefd0ca42c6a2621020014c74c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
156 schema:name Cytotoxicity, Immunologic
157 rdf:type schema:DefinedTerm
158 Nf90ba665a4aa415388c0f4cce4aea5aa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Hepatitis B Antibodies
160 rdf:type schema:DefinedTerm
161 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
162 schema:name Medical and Health Sciences
163 rdf:type schema:DefinedTerm
164 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
165 schema:name Immunology
166 rdf:type schema:DefinedTerm
167 sg:journal.1009747 schema:issn 0944-1174
168 1435-5922
169 schema:name Journal of Gastroenterology
170 rdf:type schema:Periodical
171 sg:person.01040603424.38 schema:affiliation https://www.grid.ac/institutes/grid.27476.30
172 schema:familyName Ishikawa
173 schema:givenName Tetsuya
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01040603424.38
175 rdf:type schema:Person
176 sg:person.012233615332.34 schema:affiliation https://www.grid.ac/institutes/grid.486756.e
177 schema:familyName Koike
178 schema:givenName Katsuro
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012233615332.34
180 rdf:type schema:Person
181 sg:person.01276574006.35 schema:affiliation https://www.grid.ac/institutes/grid.27476.30
182 schema:familyName Yoshioka
183 schema:givenName Kentaro
184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276574006.35
185 rdf:type schema:Person
186 sg:person.015611624262.76 schema:affiliation https://www.grid.ac/institutes/grid.27476.30
187 schema:familyName Ito
188 schema:givenName Yuji
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015611624262.76
190 rdf:type schema:Person
191 sg:person.0671521055.46 schema:affiliation https://www.grid.ac/institutes/grid.27476.30
192 schema:familyName Wakita
193 schema:givenName Takaji
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0671521055.46
195 rdf:type schema:Person
196 sg:person.0674115210.05 schema:affiliation https://www.grid.ac/institutes/grid.27476.30
197 schema:familyName Kakumu
198 schema:givenName Shinichi
199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0674115210.05
200 rdf:type schema:Person
201 sg:pub.10.1007/978-1-4612-4850-7_13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009137583
202 https://doi.org/10.1007/978-1-4612-4850-7_13
203 rdf:type schema:CreativeWork
204 sg:pub.10.1038/204998b0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038198783
205 https://doi.org/10.1038/204998b0
206 rdf:type schema:CreativeWork
207 https://app.dimensions.ai/details/publication/pub.1075488857 schema:CreativeWork
208 https://app.dimensions.ai/details/publication/pub.1078890904 schema:CreativeWork
209 https://app.dimensions.ai/details/publication/pub.1078936199 schema:CreativeWork
210 https://app.dimensions.ai/details/publication/pub.1079785412 schema:CreativeWork
211 https://app.dimensions.ai/details/publication/pub.1081652759 schema:CreativeWork
212 https://app.dimensions.ai/details/publication/pub.1081664770 schema:CreativeWork
213 https://app.dimensions.ai/details/publication/pub.1081728485 schema:CreativeWork
214 https://app.dimensions.ai/details/publication/pub.1081751737 schema:CreativeWork
215 https://doi.org/10.1016/0042-6822(73)90341-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017876073
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1016/0092-8674(83)90517-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012028100
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1016/0092-8674(86)90364-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029596455
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1016/0167-5699(88)91317-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043563368
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1016/s0092-8674(88)91043-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002057631
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1016/s0140-6736(72)90234-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008474289
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1016/s0140-6736(74)90287-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034544658
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1016/s0168-8278(87)80004-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002031509
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1073/pnas.84.4.1005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018363746
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1073/pnas.84.9.2678 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015380064
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1073/pnas.88.23.10445 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023861433
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1084/jem.158.2.303 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028788686
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1084/jem.174.6.1565 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030707982
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1111/j.1600-0676.1991.tb00500.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1046809904
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1126/science.1691527 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062500821
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1126/science.6248960 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062635849
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1159/000200935 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006432022
248 rdf:type schema:CreativeWork
249 https://www.grid.ac/institutes/grid.27476.30 schema:alternateName Nagoya University
250 schema:name Third Department of Internal Medicine, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, 466, Nagoya, Japan
251 rdf:type schema:Organization
252 https://www.grid.ac/institutes/grid.486756.e schema:alternateName The Cancer Institute Hospital
253 schema:name Department of Gene Research, Cancer Institute, Tokyo, Japan
254 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...