Evidence for IFN-β heterogeneity in a substrain of namalwa cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-04

AUTHORS

H. Borg, I. Nordensson, T. Ny, E. Lundgren

ABSTRACT

A substrain of Namalwa cells, denoted substrain B, was grown in fermentors up to the 100-L scale and was induced with Sendai virus to produce interferon (IFN). The titer of the crude IFN varied extensively between different batches; part of the variation was caused by a differential expression of IFN-α and IFN-β. More than 80% of the IFN activity was IFN-β by several criteria. A two-step purification procedure was developed and the resulting preparation had a specific activity of approximately 106 U/mg protein. The IFN-β type was found to be heterogenous, and could be separated into several components, which probably represented post-translational modifications of one molecule. More... »

PAGES

111-122

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02798543

DOI

http://dx.doi.org/10.1007/bf02798543

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029487682

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2992372


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