Horseradish peroxidase extraction and purification by aqueous two-phase partition View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-05

AUTHORS

Mar’ia V. Miranda, H’ector m. Fern’andez Lahore, Osvaldo Cascone

ABSTRACT

The effect of poly(ethyleneglycol) (PEG) molecular weight, system pH, and sodium chloride concentration on the partitioning behavior of horseradish peroxidase fromArmomcia rusticana root extract was investigated in poly(ethyleneglycol)/sodium phosphate systems. PEG molecular weight strongly affects the enzyme partition coefficient, whereas pH variation from 5.5 to 8.0 has little effect. The addition of sodium chloride (8% w/w) to a PEG 1540/phosphate system, pH 7.0, raises the peroxidase partition coefficient 13.5-fold without important changes in that of total horseradish root proteins. Moreover, these conditions allow direct homogenization of theA. rusticana roots with the selected aqueous two-phase system with the clear top phase containing over 90% of the enzyme and the purification factor being 4.8. More... »

PAGES

147-154

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02788604

DOI

http://dx.doi.org/10.1007/bf02788604

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034685445


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