Foot and mouth disease virus concentration and purification by affinity chromatography View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-12

AUTHORS

Agustin A. Navarro del Cañizo, Marcela Mazza, Rodolfo Bellinzoni, Osvaldo Cascone

ABSTRACT

Foot and mouth disease virus, (FMDV) from a crude cell lysate was purified in a single step by affinity chromatography with heparin as a ligand. The virus eluted from an Heparin-Ultrogel A4R column at 1M sodium chloride in 10 mM sodium phosphate buffer, pH 7.0, while most cell protein and albumin did so at lower concentrations of sodium chloride in the same buffer. Purity of the eluted fraction containing the virus was assessed by SDS-PAGE, HPLC, ultracentrifugation, and UV absorption spectrum. With this method, intact viral particles are recovered in high yield (over 90%) and specific virus purity increases nearly 1000-fold. The capacity of the chromatographic matrix for the virus was found to be 1.1 mg viral mass per mL of hydrated gel. More... »

PAGES

399-409

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02787811

DOI

http://dx.doi.org/10.1007/bf02787811

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1053618627

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9100360


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