Wild type and mutant forms of recombinant horseradish peroxidase C expressed inEscherichia coli View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-10

AUTHORS

E. A. Mareeva, M. A. Orlova, V. V. Doseeva, D. B. Loginov, A. G. Galkin, I. G. Gazarina, V. I. Tishok

ABSTRACT

Two horseradish peroxidase C (HRPC) mutants with substitutions in the active center, i.e., Phe41-->His and Phe143-->Glu, were compared to the wild-type recombinant enzyme expressed in Escherichia coli in terms of the enzymatic activity and stability under irradiation. Both mutations caused a significant decrease in activity, but it was still possible to follow the effect of mutations on the key steps of the reaction mechanism. Phe41 can be considered a nonpolar barrier separating histidine residues in the active center and providing a firm noncovalent binding with the highly hydrophobic porphyrin ring. The replacement of Phe41 with the ionizable His residue destabilizes the enzyme. The Phe143-->Glu replacement creates a negative charge at the entrance of the heme-binding pocket, and protects the latter from both donor substrates and free radicals. The radiolytic inactivation of the wild-type and mutant forms of recombinant HRP suggested different binding sites for iodide, 2,2'-bis(3-ethylbenzothiasoline-6-sulfonate (ABTS), guaiacol, and O-phenylene diamine. The study of kinetics and inactivation is in agreement with the direct binding of iodide to the heme porphyrin ring. The results also suggest that the ABTS binding site is less accessible than that for O-phenylene diamine. More... »

PAGES

13-23

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02785684

DOI

http://dx.doi.org/10.1007/bf02785684

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038925120

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9100342


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0305", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Organic Chemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding Sites", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Electrophoresis, Polyacrylamide Gel", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Horseradish Peroxidase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Isoenzymes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Chemical", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutagenesis, Site-Directed", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Structure-Activity Relationship", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Substrate Specificity", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Moscow State University", 
          "id": "https://www.grid.ac/institutes/grid.14476.30", 
          "name": [
            "Chemical Faculty of Moscow State University, 119899, Moscow, GSP, Russia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mareeva", 
        "givenName": "E. A.", 
        "id": "sg:person.01001032144.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001032144.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Moscow State University", 
          "id": "https://www.grid.ac/institutes/grid.14476.30", 
          "name": [
            "Chemical Faculty of Moscow State University, 119899, Moscow, GSP, Russia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Orlova", 
        "givenName": "M. A.", 
        "id": "sg:person.01261654504.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261654504.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Moscow State University", 
          "id": "https://www.grid.ac/institutes/grid.14476.30", 
          "name": [
            "Chemical Faculty of Moscow State University, 119899, Moscow, GSP, Russia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Doseeva", 
        "givenName": "V. V.", 
        "id": "sg:person.01237214736.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01237214736.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Moscow State University", 
          "id": "https://www.grid.ac/institutes/grid.14476.30", 
          "name": [
            "Chemical Faculty of Moscow State University, 119899, Moscow, GSP, Russia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Loginov", 
        "givenName": "D. B.", 
        "id": "sg:person.0604707621.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604707621.72"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Moscow State University", 
          "id": "https://www.grid.ac/institutes/grid.14476.30", 
          "name": [
            "Chemical Faculty of Moscow State University, 119899, Moscow, GSP, Russia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Galkin", 
        "givenName": "A. G.", 
        "id": "sg:person.01250123424.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250123424.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Moscow State University", 
          "id": "https://www.grid.ac/institutes/grid.14476.30", 
          "name": [
            "Chemical Faculty of Moscow State University, 119899, Moscow, GSP, Russia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gazarina", 
        "givenName": "I. G.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Moscow State University", 
          "id": "https://www.grid.ac/institutes/grid.14476.30", 
          "name": [
            "Chemical Faculty of Moscow State University, 119899, Moscow, GSP, Russia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tishok", 
        "givenName": "V. I.", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0003-9861(92)90641-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010503016"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(94)01125-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013479234"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1993.tb17654.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015085250"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0959-440x(92)90230-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016584148"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0959-440x(92)90230-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016584148"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1992.tb17077.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025195811"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2744(79)90198-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025816799"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2744(79)90198-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025816799"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj1450093", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030037461"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj1450093", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030037461"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.1994.1338", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037222824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.2.750", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040567353"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/cclm.1985.23.1.41", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044181110"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1749-6632.1994.tb47378.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051505786"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00827a015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055189681"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078746425", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1996-10", 
    "datePublishedReg": "1996-10-01", 
    "description": "Two horseradish peroxidase C (HRPC) mutants with substitutions in the active center, i.e., Phe41-->His and Phe143-->Glu, were compared to the wild-type recombinant enzyme expressed in Escherichia coli in terms of the enzymatic activity and stability under irradiation. Both mutations caused a significant decrease in activity, but it was still possible to follow the effect of mutations on the key steps of the reaction mechanism. Phe41 can be considered a nonpolar barrier separating histidine residues in the active center and providing a firm noncovalent binding with the highly hydrophobic porphyrin ring. The replacement of Phe41 with the ionizable His residue destabilizes the enzyme. The Phe143-->Glu replacement creates a negative charge at the entrance of the heme-binding pocket, and protects the latter from both donor substrates and free radicals. The radiolytic inactivation of the wild-type and mutant forms of recombinant HRP suggested different binding sites for iodide, 2,2'-bis(3-ethylbenzothiasoline-6-sulfonate (ABTS), guaiacol, and O-phenylene diamine. The study of kinetics and inactivation is in agreement with the direct binding of iodide to the heme porphyrin ring. The results also suggest that the ABTS binding site is less accessible than that for O-phenylene diamine.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf02785684", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1086169", 
        "issn": [
          "0273-2289", 
          "1559-0291"
        ], 
        "name": "Applied Biochemistry and Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1-2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "61"
      }
    ], 
    "name": "Wild type and mutant forms of recombinant horseradish peroxidase C expressed inEscherichia coli", 
    "pagination": "13-23", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "b96b4c5ad7daed9dbcf09269c7e32b1258a78ee0211f42df877877891cfacbad"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "9100342"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8208561"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf02785684"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1038925120"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf02785684", 
      "https://app.dimensions.ai/details/publication/pub.1038925120"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:29", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000370_0000000370/records_46747_00000002.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF02785684"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf02785684'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf02785684'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf02785684'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf02785684'


 

This table displays all metadata directly associated to this object as RDF triples.

187 TRIPLES      21 PREDICATES      52 URIs      31 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf02785684 schema:about N068d10d034a9471697a44db5ff04b3d3
2 N0f8248ed9b094ca78e833ea36ee45100
3 N2e7743bcc9db42889ec63addf03c62af
4 N4923bcc836d24b2796ce2368d0633dc3
5 N4a4cac5e55bb4f85b4a103fbe5b46480
6 N8dc8a5047216454fbda27358814a1011
7 Nbb3045e982694bc086c46b9ca6d9d519
8 Nc77afeebc3a646f49c0e95336f2098cd
9 Nca4b1dde527a45faab33bb1506851ac2
10 Nf13e33fc67464d53a6e637f3be5688b1
11 anzsrc-for:03
12 anzsrc-for:0305
13 schema:author N3c2fc6d668f2484c934c0314dcf02716
14 schema:citation https://app.dimensions.ai/details/publication/pub.1078746425
15 https://doi.org/10.1006/jmbi.1994.1338
16 https://doi.org/10.1016/0003-9861(92)90641-9
17 https://doi.org/10.1016/0005-2744(79)90198-0
18 https://doi.org/10.1016/0014-5793(94)01125-7
19 https://doi.org/10.1016/0959-440x(92)90230-5
20 https://doi.org/10.1021/bi00827a015
21 https://doi.org/10.1042/bj1450093
22 https://doi.org/10.1073/pnas.90.2.750
23 https://doi.org/10.1111/j.1432-1033.1992.tb17077.x
24 https://doi.org/10.1111/j.1432-1033.1993.tb17654.x
25 https://doi.org/10.1111/j.1749-6632.1994.tb47378.x
26 https://doi.org/10.1515/cclm.1985.23.1.41
27 schema:datePublished 1996-10
28 schema:datePublishedReg 1996-10-01
29 schema:description Two horseradish peroxidase C (HRPC) mutants with substitutions in the active center, i.e., Phe41-->His and Phe143-->Glu, were compared to the wild-type recombinant enzyme expressed in Escherichia coli in terms of the enzymatic activity and stability under irradiation. Both mutations caused a significant decrease in activity, but it was still possible to follow the effect of mutations on the key steps of the reaction mechanism. Phe41 can be considered a nonpolar barrier separating histidine residues in the active center and providing a firm noncovalent binding with the highly hydrophobic porphyrin ring. The replacement of Phe41 with the ionizable His residue destabilizes the enzyme. The Phe143-->Glu replacement creates a negative charge at the entrance of the heme-binding pocket, and protects the latter from both donor substrates and free radicals. The radiolytic inactivation of the wild-type and mutant forms of recombinant HRP suggested different binding sites for iodide, 2,2'-bis(3-ethylbenzothiasoline-6-sulfonate (ABTS), guaiacol, and O-phenylene diamine. The study of kinetics and inactivation is in agreement with the direct binding of iodide to the heme porphyrin ring. The results also suggest that the ABTS binding site is less accessible than that for O-phenylene diamine.
30 schema:genre research_article
31 schema:inLanguage en
32 schema:isAccessibleForFree false
33 schema:isPartOf Nbdac4731b45049778e5e719eeeb38b95
34 Nd6ccbca2463d46a3a95dafec7aab29e4
35 sg:journal.1086169
36 schema:name Wild type and mutant forms of recombinant horseradish peroxidase C expressed inEscherichia coli
37 schema:pagination 13-23
38 schema:productId N2bf9ec1636664ecfb80154ae46655d02
39 N3c42b2fbc1ec4981baddef49f8466eda
40 N3fcbbd0880f744cb94f5a3c91af68dda
41 N880a8246f6784ebca06d685ec274f75a
42 Nd5e375405c4846cf9a6521dcf9758227
43 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038925120
44 https://doi.org/10.1007/bf02785684
45 schema:sdDatePublished 2019-04-11T13:29
46 schema:sdLicense https://scigraph.springernature.com/explorer/license/
47 schema:sdPublisher N7b2fa8d6e15445d1a94e7bf47f7d2f46
48 schema:url http://link.springer.com/10.1007/BF02785684
49 sgo:license sg:explorer/license/
50 sgo:sdDataset articles
51 rdf:type schema:ScholarlyArticle
52 N068d10d034a9471697a44db5ff04b3d3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
53 schema:name Electrophoresis, Polyacrylamide Gel
54 rdf:type schema:DefinedTerm
55 N0f8248ed9b094ca78e833ea36ee45100 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
56 schema:name Escherichia coli
57 rdf:type schema:DefinedTerm
58 N2bf9ec1636664ecfb80154ae46655d02 schema:name dimensions_id
59 schema:value pub.1038925120
60 rdf:type schema:PropertyValue
61 N2e7743bcc9db42889ec63addf03c62af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
62 schema:name Mutagenesis, Site-Directed
63 rdf:type schema:DefinedTerm
64 N3999146e0ec8400785a43d2864670d68 rdf:first sg:person.01261654504.32
65 rdf:rest Nd8da91631cf7488aa53ff1412daeaecb
66 N3c2fc6d668f2484c934c0314dcf02716 rdf:first sg:person.01001032144.05
67 rdf:rest N3999146e0ec8400785a43d2864670d68
68 N3c42b2fbc1ec4981baddef49f8466eda schema:name doi
69 schema:value 10.1007/bf02785684
70 rdf:type schema:PropertyValue
71 N3fcbbd0880f744cb94f5a3c91af68dda schema:name nlm_unique_id
72 schema:value 8208561
73 rdf:type schema:PropertyValue
74 N4923bcc836d24b2796ce2368d0633dc3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Isoenzymes
76 rdf:type schema:DefinedTerm
77 N4a4cac5e55bb4f85b4a103fbe5b46480 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
78 schema:name Binding Sites
79 rdf:type schema:DefinedTerm
80 N5316fdc6815349119433c6c1b8f5a410 rdf:first sg:person.0604707621.72
81 rdf:rest Nce6bdbb935e841a2ac8cd6c09cf17997
82 N539c1672394d4eb9ae73651a20062173 schema:affiliation https://www.grid.ac/institutes/grid.14476.30
83 schema:familyName Gazarina
84 schema:givenName I. G.
85 rdf:type schema:Person
86 N7b2fa8d6e15445d1a94e7bf47f7d2f46 schema:name Springer Nature - SN SciGraph project
87 rdf:type schema:Organization
88 N865828f1a1a14f8fa404b3854d8c0659 schema:affiliation https://www.grid.ac/institutes/grid.14476.30
89 schema:familyName Tishok
90 schema:givenName V. I.
91 rdf:type schema:Person
92 N880a8246f6784ebca06d685ec274f75a schema:name pubmed_id
93 schema:value 9100342
94 rdf:type schema:PropertyValue
95 N8bffdc11e48a4b6580c65b496572ba39 rdf:first N539c1672394d4eb9ae73651a20062173
96 rdf:rest Na34732c381f644dfbe7dfd74b241d845
97 N8dc8a5047216454fbda27358814a1011 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Substrate Specificity
99 rdf:type schema:DefinedTerm
100 Na34732c381f644dfbe7dfd74b241d845 rdf:first N865828f1a1a14f8fa404b3854d8c0659
101 rdf:rest rdf:nil
102 Nbb3045e982694bc086c46b9ca6d9d519 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Recombinant Proteins
104 rdf:type schema:DefinedTerm
105 Nbdac4731b45049778e5e719eeeb38b95 schema:volumeNumber 61
106 rdf:type schema:PublicationVolume
107 Nc77afeebc3a646f49c0e95336f2098cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Models, Chemical
109 rdf:type schema:DefinedTerm
110 Nca4b1dde527a45faab33bb1506851ac2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Horseradish Peroxidase
112 rdf:type schema:DefinedTerm
113 Nce6bdbb935e841a2ac8cd6c09cf17997 rdf:first sg:person.01250123424.99
114 rdf:rest N8bffdc11e48a4b6580c65b496572ba39
115 Nd5e375405c4846cf9a6521dcf9758227 schema:name readcube_id
116 schema:value b96b4c5ad7daed9dbcf09269c7e32b1258a78ee0211f42df877877891cfacbad
117 rdf:type schema:PropertyValue
118 Nd6ccbca2463d46a3a95dafec7aab29e4 schema:issueNumber 1-2
119 rdf:type schema:PublicationIssue
120 Nd8da91631cf7488aa53ff1412daeaecb rdf:first sg:person.01237214736.47
121 rdf:rest N5316fdc6815349119433c6c1b8f5a410
122 Nf13e33fc67464d53a6e637f3be5688b1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Structure-Activity Relationship
124 rdf:type schema:DefinedTerm
125 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
126 schema:name Chemical Sciences
127 rdf:type schema:DefinedTerm
128 anzsrc-for:0305 schema:inDefinedTermSet anzsrc-for:
129 schema:name Organic Chemistry
130 rdf:type schema:DefinedTerm
131 sg:journal.1086169 schema:issn 0273-2289
132 1559-0291
133 schema:name Applied Biochemistry and Biotechnology
134 rdf:type schema:Periodical
135 sg:person.01001032144.05 schema:affiliation https://www.grid.ac/institutes/grid.14476.30
136 schema:familyName Mareeva
137 schema:givenName E. A.
138 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001032144.05
139 rdf:type schema:Person
140 sg:person.01237214736.47 schema:affiliation https://www.grid.ac/institutes/grid.14476.30
141 schema:familyName Doseeva
142 schema:givenName V. V.
143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01237214736.47
144 rdf:type schema:Person
145 sg:person.01250123424.99 schema:affiliation https://www.grid.ac/institutes/grid.14476.30
146 schema:familyName Galkin
147 schema:givenName A. G.
148 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250123424.99
149 rdf:type schema:Person
150 sg:person.01261654504.32 schema:affiliation https://www.grid.ac/institutes/grid.14476.30
151 schema:familyName Orlova
152 schema:givenName M. A.
153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261654504.32
154 rdf:type schema:Person
155 sg:person.0604707621.72 schema:affiliation https://www.grid.ac/institutes/grid.14476.30
156 schema:familyName Loginov
157 schema:givenName D. B.
158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604707621.72
159 rdf:type schema:Person
160 https://app.dimensions.ai/details/publication/pub.1078746425 schema:CreativeWork
161 https://doi.org/10.1006/jmbi.1994.1338 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037222824
162 rdf:type schema:CreativeWork
163 https://doi.org/10.1016/0003-9861(92)90641-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010503016
164 rdf:type schema:CreativeWork
165 https://doi.org/10.1016/0005-2744(79)90198-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025816799
166 rdf:type schema:CreativeWork
167 https://doi.org/10.1016/0014-5793(94)01125-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013479234
168 rdf:type schema:CreativeWork
169 https://doi.org/10.1016/0959-440x(92)90230-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016584148
170 rdf:type schema:CreativeWork
171 https://doi.org/10.1021/bi00827a015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055189681
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1042/bj1450093 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030037461
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1073/pnas.90.2.750 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040567353
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1111/j.1432-1033.1992.tb17077.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1025195811
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1111/j.1432-1033.1993.tb17654.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1015085250
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1111/j.1749-6632.1994.tb47378.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1051505786
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1515/cclm.1985.23.1.41 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044181110
184 rdf:type schema:CreativeWork
185 https://www.grid.ac/institutes/grid.14476.30 schema:alternateName Moscow State University
186 schema:name Chemical Faculty of Moscow State University, 119899, Moscow, GSP, Russia
187 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...