Isolation and characterization of a newly identified type II restriction endonuclease from a local streptomyces sp. in Taiwan View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-05

AUTHORS

Kuang-Yu Hu, Jia-An Wuu, Ming-Ching Kao, Yu-Tien Liu, Shou-Hsiung Pai

ABSTRACT

Streptomyces chusanensis ZS-2, isolated from a soil sample in Chusan in Taiwan, was found to produce a new Type II restriction endonuclease. This restriction enzyme was designated as SchI. The purified enzyme was characterized as having a subunit mol wt of 28 kDa, and was apparently free from exonuclease activities. It cleaves the phosphodiester bond between the fourth C and the fifth G on the 5’-CCGCGG-3’ sequence of DNAs, leaving a 2-nucleotide protruding end at its 3’ site. This data suggests that SchI is an isoschizomer of SacII. In addition, based on the comparison between SchI and SacII regarding reaction parameters, it seems that SchI is a better choice of restriction enzyme for genetic analysis and mapping. More... »

PAGES

231-241

References to SciGraph publications

  • 1995-06. Isolation of SagI, a new HaeIII isoschizomer from Streptococcus agalactiae in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02785658

    DOI

    http://dx.doi.org/10.1007/bf02785658

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1051234267

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/9779579


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