Improved high-throughput sunflower and cotton genomic DNA extraction and PCR fidelity View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-03

AUTHORS

Erin C. Horne, Siva P. Kumpatla, Koni A. Patterson, Manju Gupta, Steven A. Thompson

ABSTRACT

The extraction of high-quality genomic DNA for PCR amplification from sunflower (Helianthus annuus) and cotton (Gossypium spp.) is challenging because of the presence of polysaccharides, secondary metabolites, and polyphenolics in the tissues. A high-throughput DNA extraction protocol was needed in our laboratory for simple sequence repeats (SSR)-marker screening and other molecular analyses that do not require organic extraction steps of phenol or chloroform. Here we describe 2 improved highthroughput protocols for DNA extraction and in-PCR modification that result in successful PCR amplification of sunflower and cotton. While the sunflower DNA extraction protocol uses reducing agents such as sodium metabisulfite and dithiothreitol (DTT), the cotton protocol uses polyvinylpyrrolidone (PVP) in PCR reactions and reducing agents in the DNA extraction procedure. More... »

PAGES

83-84

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02773352

DOI

http://dx.doi.org/10.1007/bf02773352

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016741379


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