Ontology type: schema:ScholarlyArticle
2004-03
AUTHORSErin C. Horne, Siva P. Kumpatla, Koni A. Patterson, Manju Gupta, Steven A. Thompson
ABSTRACTThe extraction of high-quality genomic DNA for PCR amplification from sunflower (Helianthus annuus) and cotton (Gossypium spp.) is challenging because of the presence of polysaccharides, secondary metabolites, and polyphenolics in the tissues. A high-throughput DNA extraction protocol was needed in our laboratory for simple sequence repeats (SSR)-marker screening and other molecular analyses that do not require organic extraction steps of phenol or chloroform. Here we describe 2 improved highthroughput protocols for DNA extraction and in-PCR modification that result in successful PCR amplification of sunflower and cotton. While the sunflower DNA extraction protocol uses reducing agents such as sodium metabisulfite and dithiothreitol (DTT), the cotton protocol uses polyvinylpyrrolidone (PVP) in PCR reactions and reducing agents in the DNA extraction procedure. More... »
PAGES83-84
http://scigraph.springernature.com/pub.10.1007/bf02773352
DOIhttp://dx.doi.org/10.1007/bf02773352
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1016741379
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Department of Trait Genetics and Technologies, Dow AgroSciences LLC, Building 306/C2, 9330 Zionsville Road, 46268, Indianapolis, IN",
"id": "http://www.grid.ac/institutes/grid.418574.b",
"name": [
"Department of Trait Genetics and Technologies, Dow AgroSciences LLC, Building 306/C2, 9330 Zionsville Road, 46268, Indianapolis, IN"
],
"type": "Organization"
},
"familyName": "Horne",
"givenName": "Erin C.",
"id": "sg:person.011456762760.50",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011456762760.50"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Trait Genetics and Technologies, Dow AgroSciences LLC, Building 306/C2, 9330 Zionsville Road, 46268, Indianapolis, IN",
"id": "http://www.grid.ac/institutes/grid.418574.b",
"name": [
"Department of Trait Genetics and Technologies, Dow AgroSciences LLC, Building 306/C2, 9330 Zionsville Road, 46268, Indianapolis, IN"
],
"type": "Organization"
},
"familyName": "Kumpatla",
"givenName": "Siva P.",
"id": "sg:person.01032130304.58",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01032130304.58"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Trait Genetics and Technologies, Dow AgroSciences LLC, Building 306/C2, 9330 Zionsville Road, 46268, Indianapolis, IN",
"id": "http://www.grid.ac/institutes/grid.418574.b",
"name": [
"Department of Trait Genetics and Technologies, Dow AgroSciences LLC, Building 306/C2, 9330 Zionsville Road, 46268, Indianapolis, IN"
],
"type": "Organization"
},
"familyName": "Patterson",
"givenName": "Koni A.",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Trait Genetics and Technologies, Dow AgroSciences LLC, Building 306/C2, 9330 Zionsville Road, 46268, Indianapolis, IN",
"id": "http://www.grid.ac/institutes/grid.418574.b",
"name": [
"Department of Trait Genetics and Technologies, Dow AgroSciences LLC, Building 306/C2, 9330 Zionsville Road, 46268, Indianapolis, IN"
],
"type": "Organization"
},
"familyName": "Gupta",
"givenName": "Manju",
"id": "sg:person.01243034607.23",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01243034607.23"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Trait Genetics and Technologies, Dow AgroSciences LLC, Building 306/C2, 9330 Zionsville Road, 46268, Indianapolis, IN",
"id": "http://www.grid.ac/institutes/grid.418574.b",
"name": [
"Department of Trait Genetics and Technologies, Dow AgroSciences LLC, Building 306/C2, 9330 Zionsville Road, 46268, Indianapolis, IN"
],
"type": "Organization"
},
"familyName": "Thompson",
"givenName": "Steven A.",
"id": "sg:person.01311150007.81",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01311150007.81"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/bf02772108",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007521984",
"https://doi.org/10.1007/bf02772108"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02669265",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010583798",
"https://doi.org/10.1007/bf02669265"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02670470",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010735463",
"https://doi.org/10.1007/bf02670470"
],
"type": "CreativeWork"
}
],
"datePublished": "2004-03",
"datePublishedReg": "2004-03-01",
"description": "The extraction of high-quality genomic DNA for PCR amplification from sunflower (Helianthus annuus) and cotton (Gossypium spp.) is challenging because of the presence of polysaccharides, secondary metabolites, and polyphenolics in the tissues. A high-throughput DNA extraction protocol was needed in our laboratory for simple sequence repeats (SSR)-marker screening and other molecular analyses that do not require organic extraction steps of phenol or chloroform. Here we describe 2 improved highthroughput protocols for DNA extraction and in-PCR modification that result in successful PCR amplification of sunflower and cotton. While the sunflower DNA extraction protocol uses reducing agents such as sodium metabisulfite and dithiothreitol (DTT), the cotton protocol uses polyvinylpyrrolidone (PVP) in PCR reactions and reducing agents in the DNA extraction procedure.",
"genre": "article",
"id": "sg:pub.10.1007/bf02773352",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1124107",
"issn": [
"0735-9640",
"1556-469X"
],
"name": "Plant Molecular Biology Reporter",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "22"
}
],
"keywords": [
"DNA extraction protocol",
"high-throughput DNA extraction protocol",
"high-quality genomic DNA",
"simple sequence repeats",
"PCR amplification",
"DNA extraction",
"genomic DNA extraction",
"sequence repeats",
"DNA extraction procedure",
"successful PCR amplification",
"extraction protocol",
"organic extraction step",
"PCR fidelity",
"genomic DNA",
"secondary metabolites",
"molecular analysis",
"PCR reaction",
"presence of polysaccharides",
"sunflower",
"protocol",
"marker screening",
"cotton",
"dithiothreitol",
"repeats",
"amplification",
"DNA",
"extraction step",
"polysaccharides",
"metabolites",
"extraction procedure",
"tissue",
"modification",
"fidelity",
"agents",
"polyphenolics",
"extraction",
"screening",
"presence",
"phenol",
"reaction",
"step",
"analysis",
"chloroform",
"laboratory",
"sodium metabisulfite",
"procedure",
"metabisulfite"
],
"name": "Improved high-throughput sunflower and cotton genomic DNA extraction and PCR fidelity",
"pagination": "83-84",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1016741379"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/bf02773352"
]
}
],
"sameAs": [
"https://doi.org/10.1007/bf02773352",
"https://app.dimensions.ai/details/publication/pub.1016741379"
],
"sdDataset": "articles",
"sdDatePublished": "2022-08-04T16:55",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_388.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/bf02773352"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf02773352'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf02773352'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf02773352'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf02773352'
This table displays all metadata directly associated to this object as RDF triples.
143 TRIPLES
21 PREDICATES
75 URIs
64 LITERALS
6 BLANK NODES