Construction of a sugar beet BAC library from a hybrid with diverse traits View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-03

AUTHORS

J. Mitchell McGrath, R. Scott Shaw, Benildo G. de los Reyes, John J. Weiland

ABSTRACT

A bacterial artificial chromosome (BAC) library of the 750-Mbp sugar beet genome represented in hybrid US H20 was constructed fromHind III-digested DNA, with an average insert size of 120 kbp. US H20 is a variety grown in the eastern United States. It exhibits heterosis for emergence and yield, presumably because of its hybridity between eastern and western US germplasm sources. Filter arrays were used to assess the abundance and distribution of particular nucleotide sequences. An rRNA gene probe found that 1.2% of the library carried sequences similar to these highly repetitive and conserved sequences. A simple sequence repeat element (CA)8 thought to be predominantly distributed throughout centromere regions of all chromosomes was present in 1.7% of clones. For more than half of the 28 randomly chosen expressed sequence tags (ESTs) used as probes, a higher-than-expected number of single-copy hybridization signals was observed. Assuming 6× genome coverage, this suggests that many duplicate genes exist in the beet genome. More... »

PAGES

23-28

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02773345

DOI

http://dx.doi.org/10.1007/bf02773345

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020097953


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