Subtractive hybridization of cDNA from small amounts of plant tissue View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-08

AUTHORS

Tatjana Heinrich, Stewart Washer, John Marshall, Michael G. K. Jones, Robert H. Potter

ABSTRACT

A subtractive hybridization method is described that allows the generation of a subtractive gene library from small amounts of plant or other eukaryotic tissues. The method uses paramagnetic oligo-dT beads to capture poly-adenylated mRNA and to synthesize the complementary cDNA on a solid support. The use of magnetic beads facilitates the change of reaction buffers and the removal of primers and minimizes yield losses. Subtracted material obtained from this method can either be cloned directly or used to screen a specific library. More... »

PAGES

7-12

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02762335

DOI

http://dx.doi.org/10.1007/bf02762335

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026444056

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9327393


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