In situ localization of PCR-amplified DNA and cDNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-08

AUTHORS

Gerard J. Nuovo

ABSTRACT

Combining the high sensitivity of PCR with the cell localizing ability of in situ hybridization allows for the reproducible detection of low copy targets in intact cells. This article describes several key variables that include fixation, protease digestion, the hot start maneuver, stringency, and, for RNA analysis, DNase digestion that are important to successful in situ PCR. Also stressed is the importance of performing and interpreting controls with each experiment. Important controls include omission of key components, use of samples known either to contain or lack the target of interest and, most importantly, the in-built controls invariably present in the heterogeneous component of any given tissue type. More... »

PAGES

49-62

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02745862

DOI

http://dx.doi.org/10.1007/bf02745862

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048286505

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9779422


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