Mini-prep method suitable for a plant breeding program View Full Text


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Article Info

DATE

1996-09

AUTHORS

K. M. Haymes

ABSTRACT

Plant breeders need a nondestructive and inexpensive protocol to screen large numbers of plants early after sowing. Isolation of DNA represents one of the limiting steps in this process: normally a person is capable of isolating 25 to 50 samples per day. To speed up the isolation of DNA in marker-assisted plant breeding programs, a CTAB mini-prep was modified into an inexpensive and nondestructive procedure. With this protocol a single individual can isolate 200 to 250 samples per day of high-quality DNA that is immediately suitable for testing by PCR. The amounts of DNA isolated are sufficient for about 150 PCR amplifications. These improvements are achieved by eliminating time-consuming and noncritical steps in the standard protocol, such as extensive grinding, washing with ethanol, and treatment with RNAse A. More... »

PAGES

280-284

References to SciGraph publications

  • 1990-11. A rapid and inexpensive method for isolation of total DNA from dehydrated plant tissue in PLANT MOLECULAR BIOLOGY REPORTER
  • 1991-09. Direct amplification of plant genomic DNA from leaf and root pieces using PCR in PLANT MOLECULAR BIOLOGY
  • 1990-02. Isolation of DNA from plants high in polyphenolics in PLANT MOLECULAR BIOLOGY REPORTER
  • 1983-09. A plant DNA minipreparation: Version II in PLANT MOLECULAR BIOLOGY REPORTER
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02671664

    DOI

    http://dx.doi.org/10.1007/bf02671664

    DIMENSIONS

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