A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis View Full Text


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Article Info

DATE

1993-06

AUTHORS

Andrew H. Paterson, Curt L. Brubaker, Jonathan F. Wendel

ABSTRACT

Extraction of high-quality genomic DNA fromGossypium (cotton) species is difficult due to high levels of polysaccharide, oxidizable quinones, and other interfering substances. We describe a procedure that consistently permits isolation of cotton genomic DNA of satisfactory size and quality for RFLP and PCR analysis, as well as for most routine cloning applications. Several antioxidants, phenol-binding reagents, and phenol oxidase inhibitors are used throughout the procedure, and most polysaccharides are eliminated early in the procedure by isolation of nuclei. More... »

PAGES

122-127

References to SciGraph publications

  • 1990-02. Isolation of DNA from plants high in polyphenolics in PLANT MOLECULAR BIOLOGY REPORTER
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02670470

    DOI

    http://dx.doi.org/10.1007/bf02670470

    DIMENSIONS

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