Isolation of plant DNA: A fast, inexpensive, and reliable method View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-02

AUTHORS

Pierre Guillemaut, Laurence Maréchal-Drouard

ABSTRACT

We describe here a simple method to isolate DNA of high molecular weight from a wide variety of plant materials, such as trees, herbaceous plants, cell suspension cultures, calli, seeds, dried embryos, ferns and lichens. The crucial step of the extraction is the use of an acidic extraction medium. When necessary, the sample was separated on a fast RPC-5 column providing us with highly purified DNA suitable not only for restriction endonuclease analyses but also for PCR experiments, RLFP analyses, or detection of adducts. More... »

PAGES

60-65

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02669265

DOI

http://dx.doi.org/10.1007/bf02669265

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010583798


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