Large-scale mapping using high-molecular-weight DNA fromP. vulgaris View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-05

AUTHORS

Francine Creusot, Mireille Sevignac, Michel Dron

ABSTRACT

The application of megabase methods is essential for positional cloning of genes for which no products have been characterized. We have set about the isolation of genes conferring resistance to pathogens in the french beanPhaseolus vulgaris. We describe here the isolation of high-molecular-weight DNA, pulse-field gel electrophoresis and large-scale mapping of thePal2 locus inP. vulgaris. We describe the construction of such a map by embedding DNA isolated from leaf nuclei in agarose plugs, restricting it with rare cutters and subjecting the DNA to pulse-field gel electrophoresis. More... »

PAGES

207-218

References to SciGraph publications

  • 1989-05. Mapping of theHor2 locus in barley by pulsed field gel electrophoresis in CARLSBERG RESEARCH COMMUNICATIONS
  • 1991-05. Construction and characterization of a yeast artificial chromosome library of Arabidopsis which is suitable for chromosome walking in MOLECULAR GENETICS AND GENOMICS
  • 1989-02. Pulsed field gel electrophoresis and physical mapping of large DNA fragments in the Tm-2a region of chromosome 9 in tomato in MOLECULAR GENETICS AND GENOMICS
  • 1989-04. Phenylalanine ammonia-lyase gene organization and structure in PLANT MOLECULAR BIOLOGY
  • 1981-08. Sequence specificity of methylation in higher plant DNA in NATURE
  • 1990-04. Isolation of single-copy-sequence clones from a yeast artificial chromosome library of randomly-sheared Arabidopsis thaliana DNA in PLANT MOLECULAR BIOLOGY
  • 1989-03. Preparation of megabase-sized tomato DNA and separation of large restriction fragments by field inversion gel electrophoresis (FIGE) in PLANT MOLECULAR BIOLOGY
  • 1991-04. Construction of physical maps of the Hor1 locus of two barley cultivars by pulsed field gel electrophoresis in MOLECULAR GENETICS AND GENOMICS
  • 1990-11. Analysis of rice (Oryza sativa L.) genome using pulsed-field gel electrophoresis and rare-cutting restriction endonucleases in PLANT MOLECULAR BIOLOGY REPORTER
  • 1990-01. Methylated and undermethylated rDNA repeats are interspersed at random in two higher plant species in PLANT MOLECULAR BIOLOGY
  • 1990-05. The isolation of high molecular weight DNA from wheat, barley and rye for analysis by pulse-field gel electrophoresis in PLANT MOLECULAR BIOLOGY
  • 1989-05. Transgenic Plants of Lettuce (Lactuca sativa) Obtained Through Electroporation of Protoplasts in NATURE BIOTECHNOLOGY
  • Journal

    TITLE

    Plant Molecular Biology Reporter

    ISSUE

    2

    VOLUME

    10

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf02668348

    DOI

    http://dx.doi.org/10.1007/bf02668348

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1011269345


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