High-frequency transformation ofArabidopsis thaliana byAgrobacterium tumefaciens View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-05

AUTHORS

Michael C. Clarke, Wenbin Wei, Keith Lindsey

ABSTRACT

Incorporation of 5 mg/L silver thiosulphate into media for seed germination and callus induction, as used in the transformation protocol originally described by Valvekens et al. (1988), was found to increase the frequency of regeneration of transformants ofArabidopsis thaliana ecotypes C24 and Landsbergerecta by at least 10- to 100-fold. Other factors, such as density of the bacterial inoculation culture, density of the root explants and duration of bacteria-plant cocultivation period, were also found to influence the efficiency of recovery of transformants. More... »

PAGES

178-189

Journal

TITLE

Plant Molecular Biology Reporter

ISSUE

2

VOLUME

10

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02668345

DOI

http://dx.doi.org/10.1007/bf02668345

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040544940


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