The use of thidiazuron in tissue culture View Full Text


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Article Info

DATE

1993-04

AUTHORS

Chin-Yi Lu

ABSTRACT

Thidiazuron (N-phenyl-N’-1,2,3-thiadiazol-5-ylurea) was first reported to have cytokinin activity in 1982. Since then, thidiazuron has been used successfully in vitro to induce adventitious shoot formation and to promote axillary shoot proliferation. Thidiazuron is especially effective with recalcitrant woody species. Shoot numbers produced on medium containing thidiazuron are equivalent to or greater than numbers initiated on medium with purine-type cytokinins. Low concentrations of thidiazuron (0.0022 to 0.088 mg/liter) are effective for micropropagation. Prolonged exposure to thidiazuron should be avoided, as this may cause hyperhydricity, abnormal shoot morphology, or problems in rooting. More... »

PAGES

92-96

References to SciGraph publications

  • 1991-05. Adventitious shoot regeneration from in vitro leaves of several pear cultivars (Pyrus communis L.) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1991-11. Adventitious bud regeneration from leaf expiants of the shrubby ornamental honeysuckle, Lonicera nitida Wils. cv. ‘Maigrün’: effects of thidiazuron and 2,3,5-triiodobenzoic acid in PLANT CELL REPORTS
  • 1992-08. Reconsideration of the term ‘vitrification’ as used in micropropagation in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1991-09. Agrobacterium-Mediated Transformation of Carnation (Dianthus caryophyllus L.) in NATURE BIOTECHNOLOGY
  • 1990-05. The effect of in vitro pretreatments on subsequent shoot organogenesis from excised Rubus and Malus leaves in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1991-09. Agrobacterium-Mediated Transformation of Plum (Prunus domestica L.) Hypocotyl Slices and Regeneration of Transgenic Plants in NATURE BIOTECHNOLOGY
  • 1989-10. Plant regeneration from cotyledons of Prunus persica, Prunus domestica, and Prunus cerasus in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1991-08. Plantlet regeneration from cultured leaves of Cydonia oblonga L. (quince) in PLANT CELL REPORTS
  • 1989-03. Adventitious shoot regeneration from leaf tissue of three pear (Pyrus sp.) cultivars in vitro in PLANT CELL REPORTS
  • 1992-08. Adventitious shoot induction and somatic embryogenesis with intact seedlings of several hybrid seed geranium (Pelargonium x hortorum Bailey) varieties in PLANT CELL REPORTS
  • 1992-06. Induction by thidiazuron of somatic embryogenesis in intact seedlings of peanut in PLANTA
  • 1992-09. Regeneration of shoots from embryo hypocotyls of common ash (Fraxinus excelsior) in PLANT CELL REPORTS
  • 1991-11. Plant regeneration from stem and petal of carnation (Dianthus caryophyllus L.) in PLANT CELL REPORTS
  • 1990-03. Effect of thidiazuron, light fluence rates and kanamycin on in vitro shoot organogenesis from excised Rubus cotyledons and leaves in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1989-09. Factors influencing shoot regeneration from cotyledonary explants ofCucumis melo in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1992-02. Regeneration in Phaseolus vulgaris L.: High-frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron in PLANTA
  • 1991-12. Adventitious shoot regeneration from leaf explants of tissue cultured and greenhouse-grown raspberry in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1991-06. A liquid cytokinin pulse induces adventitious shoot formation from Douglas-fir cotyledons in PLANT CELL REPORTS
  • 1991-12. A comparison of BA, zeatin and thidiazuron for adventitious bud formation from Picea glauca embryos and epicotyl explants in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1989-01. Adventitions shoot formation on excised leaves of in vitro grown shoots of apple cultivars in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
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    http://scigraph.springernature.com/pub.10.1007/bf02632259

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