A simple method for the recovery of multiple fertile plants from individual somatic embryos of soybean [Glycine max (L.) merrill] View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-07

AUTHORS

M. S. Wright, K. L. Launis, R. Novitzky, J. H. Duesing, C T. Harms

ABSTRACT

We describe a method for obtaining and proliferating multiple, fertile plants from somatic embryos of several experimental and commercial soybean varieties. Shoot-bud cultures were initiated by placing cotyledonary and torpedo-stage somatic embryos derived from immature seedling cotyledons onto Cheng’s basal medium (CBO) containing 0.5 to 2.5 mg/liter 6-benzyladenine (6-BA). Prolific masses of adventitious shoots were produced within 6 to 18 wk. These cultures can be propagated indefinitely with regular subcultures to CBO containing 0.5 mg/liter 6-BA. Individual shoots were separated from the clusters and were rooted on CBO medium without exogenous growth regulators. By this method any number of plants can be produced from individual somatic embryos. The risk of losing valuable genotypes (e.g., derived from in vitro selection or transformation) due to inefficient embryo germination and embryo-to-plant conversion is thus greatly reduced. Plants were established in the greenhouse and progenies were field tested. Progenies from shoot-bud culture-derived plants showed no somaclonal variation for the seven recessive marker traits or quantitative agronomic characters evaluated under field conditions. More... »

PAGES

153-157

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02632200

DOI

http://dx.doi.org/10.1007/bf02632200

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045377617


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