Standardization of spectrophotometric methods for determination of polyunsaturated fatty acids using pure natural acids View Full Text


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Article Info

DATE

1952-07

AUTHORS

B. A. Brice, M. L. Swain, S. F. Herb, P. L. Nichols, R. W. Riemenschneider

ABSTRACT

Spectrophotometric methods of analysis for the polyunsaturated constituents of oils and fats have been carefully restandardized for several conditions of alkali-isomerization, using purified methyl esters of linoleic, linolenic, and arachidonic acids prepared by physical rather than by chemical means. A number of vegetable oil and animal fat samples were subjected to spectrophotometric analysis, and the results based on natural and on bromination-debromination fatty acid standards were compared. The natural fatty acid standards lead to significantly higher accuracy and their use in the spectrophotometric analysis of natural fatty materials is strongly recommended. Results obtained under different conditions of isomerization were in satisfactory agreement. An isomerization time of 45 minutes is recommended rather than 25 or 30 minutes. The glycerol-air technique is preferred for general use because of its simplicity and high precision. The ethylene-glycol-nitrogen technique is a close second choice because of the greater transparency of reagent blanks. More... »

PAGES

279-287

References to SciGraph publications

  • 1948-01. Report of the Spectroscopy Committee in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1946-01. Spectral properties of linoleic and linolenic acids prepared by debromination in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1951-07. Studies of the spectral characteristics of alkali-isomerized autoxidized fatty acids in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1951-02. Isolation of natural arachidonic acid as its methyl ester in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1945-09. Spectrophotometric determination of small proportions of polyunsaturated constituents in fatty materials in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1945-10. Spectrophotometric estimation of soybean oil in admixture with cottonseed and peanut oils in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1950-09. Kinetics of alkali isomerization of linoleic, linolenic, and arachidonic acids in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1946-05. Applied ultraviolet spectrophotometry of fats and oils in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1949-06. Formation of traces of conjugated tetraenoic and trienoic constituents in autoxidized linolenic and linoleic acids and vegetable oils during alkali-isomerization in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1949-07. Isolation of pure natural linoleic and linolenic acids as their methyl esters by adsorption fractionation on silicic acid in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1945-06. Fatty acid analyses of known mixtures of purified methyl esters in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
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    URI

    http://scigraph.springernature.com/pub.10.1007/bf02631475

    DOI

    http://dx.doi.org/10.1007/bf02631475

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1013043301


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