Isolation and culture of soybean hypocotyl protoplasts View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-06

AUTHORS

N. Hammatt, M. R. Davey

ABSTRACT

Protoplasts were isolated seedling hypocotyls of soybean (Glycine max), and cultured in both liquid and agarose-solidified, modified K8P medium. Nuclear staining revealed that only 2% of protoplasts lacked a nucleus, 93% contained a single nucleus, and 5% contained more than one. Maximum protoplast yields and subsequent division frequencies, in liquid medium, were obtained from 5 days-old seedlings. Maximum division frequencies (54%) were obtained from hypocotyl protoplasts plated at a density of 5×104 ml−1. Using different osmolality reduction régimes for liquid cultures, hypocotyl protoplasts developed into green, nodular callus, similar to that which has previously given rise to shoot buds in perennialGlycine species. This tissue, however, did not produce shoot buds in soybean. More... »

PAGES

601-604

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02629097

DOI

http://dx.doi.org/10.1007/bf02629097

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002491375


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