Plant regeneration fromBrassicanigra (L.) Koch stem protoplasts View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-06

AUTHORS

Phan V. Chuong, K. P. Pauls, W. D. Beversdorf

ABSTRACT

Protoplasts ofBrassica nigra (L.) Koch were isolated from stem peels of bolting racemes and cultured in 1.5 ml of VN1 liquid medium. The protoplasts in the liquid medium were plated on top of half strength MS medium supplemented with 400 mg/liter glutamine, 15 mg/liter glutathione, 50 mg/literl-serine, 0.25 mg/liter 6-benzylaminopurine, 0.5 mg/liter 2,4-dichlorophenoxyacetic acid, 1.5% sucrose, and 5% mannitol, pH, 5.7, solidified with 0.3% agarose. Ten percent of calli obtained from the protoplasts developed into plantlets within 4 wk after transfer onto 2N regeneration medium which contains MS salts plus 200 mg/liter casein hydrolysate, 0.625 mg/liter 6-benzylaminopurine, 0.625 mg/liter kinetin, 0.625 mg/liter 6-(γ,γ-dimethylallylamino)-purine, 0.625 mg/liter zeatin, 0.5 mg/liter 1-naphthaleneacetic acid, 1.5% sucrose, and 0.4% agarose. THis is the first report of plant regeneration fromB. nigra protoplasts. More... »

PAGES

449-452

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02623862

DOI

http://dx.doi.org/10.1007/bf02623862

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000821421


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