Growth and hepatospecific gene expression of human hepatoma cells in a defined medium View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-05

AUTHORS

G. J. Darlington, J. H. Kelly, G. J. Buffone

ABSTRACT

The production of albumin, α-fetoprotein (AFP), and α-1 antitrypsin has been compared among human hepatoma cells cultured in medium containing serum, medium containing hormones and growth factors, and a basal medium containing selenium as the only supplement. Growth is sustained in all three media, and the expression of all three proteins was maintained for over 4 mo. in the various media. However, the quantitative production of albumin and AFP were dramatically different in the three media. Two hormones, insulin and triiodothyronine, influenced the level of secreted proteins. Triiodothyronine increases the amount of secreted albumin whereas insulin at 10 μg/ml reduced the level of total secreted protein. More... »

PAGES

349-354

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02620991

DOI

http://dx.doi.org/10.1007/bf02620991

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034518870

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/3034851


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