Selective elimination of fibroblasts from cultures of normal human melanocytes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1984-05

AUTHORS

Ruth Halaban, Francis D. Alfano

ABSTRACT

The main obstacle to establishing pure normal human melanocytes in vitro is contamination of the cultures by fibroblasts. The obstacle can be overcome by selective destruction of fibroblasts with geneticin (G418 sulfate). Treatment of mixed cultures with this drug at a concentration of 100 μg/ml for two days results in pure cultures of normal human melanocytes. More... »

PAGES

447-450

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf02619590

DOI

http://dx.doi.org/10.1007/bf02619590

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1008156207

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6724622


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Division", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Separation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Culture Media", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Culture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fibroblasts", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gentamicins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kinetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Melanocytes", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Dermatology, Yale University School of Medicine, 333 Cedar Street, 06510, New Haven, Connecticut", 
          "id": "http://www.grid.ac/institutes/grid.47100.32", 
          "name": [
            "Department of Dermatology, Yale University School of Medicine, 333 Cedar Street, 06510, New Haven, Connecticut"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Halaban", 
        "givenName": "Ruth", 
        "id": "sg:person.053601122.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.053601122.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Dermatology, Yale University School of Medicine, 333 Cedar Street, 06510, New Haven, Connecticut", 
          "id": "http://www.grid.ac/institutes/grid.47100.32", 
          "name": [
            "Department of Dermatology, Yale University School of Medicine, 333 Cedar Street, 06510, New Haven, Connecticut"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Alfano", 
        "givenName": "Francis D.", 
        "id": "sg:person.0755076137.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755076137.76"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1984-05", 
    "datePublishedReg": "1984-05-01", 
    "description": "The main obstacle to establishing pure normal human melanocytes in vitro is contamination of the cultures by fibroblasts. The obstacle can be overcome by selective destruction of fibroblasts with geneticin (G418 sulfate). Treatment of mixed cultures with this drug at a concentration of 100 \u03bcg/ml for two days results in pure cultures of normal human melanocytes.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf02619590", 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2465828", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1312285", 
        "issn": [
          "0883-8364"
        ], 
        "name": "In Vitro Cellular & Developmental Biology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "20"
      }
    ], 
    "keywords": [
      "normal human melanocytes", 
      "human melanocytes", 
      "selective destruction", 
      "days results", 
      "melanocytes", 
      "fibroblasts", 
      "selective elimination", 
      "drugs", 
      "treatment", 
      "vitro", 
      "culture", 
      "destruction", 
      "elimination", 
      "concentration", 
      "geneticin", 
      "pure culture", 
      "main obstacles", 
      "mixed culture", 
      "results", 
      "obstacles", 
      "contamination"
    ], 
    "name": "Selective elimination of fibroblasts from cultures of normal human melanocytes", 
    "pagination": "447-450", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1008156207"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf02619590"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "6724622"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf02619590", 
      "https://app.dimensions.ai/details/publication/pub.1008156207"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-10-01T06:27", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/article/article_172.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf02619590"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf02619590'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf02619590'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf02619590'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf02619590'


 

This table displays all metadata directly associated to this object as RDF triples.

126 TRIPLES      20 PREDICATES      56 URIs      48 LITERALS      16 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf02619590 schema:about N144f638afd0046bd8a33194c3e977c2e
2 N1562c37b5a464fb9a960b0ae235cfbba
3 N4998a17796ec4533890f68c5385e0e91
4 N9eba758a06f0409dae423fa596fa1011
5 Na7fa72e628104812885fb56bbc44ca72
6 Na80482b380d24f159919d235a5628b2f
7 Nb400efc170334f12b49630fee3ccbb92
8 Nb87b0506e6284d43b2aea7b453ee4709
9 Nc66957ae235843b3ad218ee9fb2e07ed
10 anzsrc-for:06
11 anzsrc-for:0601
12 schema:author N2d93224777b14aab82a2c5214e0de8d4
13 schema:datePublished 1984-05
14 schema:datePublishedReg 1984-05-01
15 schema:description The main obstacle to establishing pure normal human melanocytes in vitro is contamination of the cultures by fibroblasts. The obstacle can be overcome by selective destruction of fibroblasts with geneticin (G418 sulfate). Treatment of mixed cultures with this drug at a concentration of 100 μg/ml for two days results in pure cultures of normal human melanocytes.
16 schema:genre article
17 schema:isAccessibleForFree false
18 schema:isPartOf Na1f66a75f2124f3bae01caefcb23f2f6
19 Nc7da9e7d13814f81bde45506f9fb8646
20 sg:journal.1312285
21 schema:keywords concentration
22 contamination
23 culture
24 days results
25 destruction
26 drugs
27 elimination
28 fibroblasts
29 geneticin
30 human melanocytes
31 main obstacles
32 melanocytes
33 mixed culture
34 normal human melanocytes
35 obstacles
36 pure culture
37 results
38 selective destruction
39 selective elimination
40 treatment
41 vitro
42 schema:name Selective elimination of fibroblasts from cultures of normal human melanocytes
43 schema:pagination 447-450
44 schema:productId N30430ffd52d6429cb93d62f24d7e6c7a
45 N5f93bd12f54742b4b36d842dbe0802a1
46 Nc7ef7ccfbeb446a3bb901ae8184f85d6
47 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008156207
48 https://doi.org/10.1007/bf02619590
49 schema:sdDatePublished 2022-10-01T06:27
50 schema:sdLicense https://scigraph.springernature.com/explorer/license/
51 schema:sdPublisher Ne850655ecb0649648e7634596efbcb2b
52 schema:url https://doi.org/10.1007/bf02619590
53 sgo:license sg:explorer/license/
54 sgo:sdDataset articles
55 rdf:type schema:ScholarlyArticle
56 N144f638afd0046bd8a33194c3e977c2e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
57 schema:name Culture Media
58 rdf:type schema:DefinedTerm
59 N1562c37b5a464fb9a960b0ae235cfbba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
60 schema:name Culture Techniques
61 rdf:type schema:DefinedTerm
62 N2d93224777b14aab82a2c5214e0de8d4 rdf:first sg:person.053601122.01
63 rdf:rest N3b2547b3f1fb46e5b43c0c8261681126
64 N30430ffd52d6429cb93d62f24d7e6c7a schema:name pubmed_id
65 schema:value 6724622
66 rdf:type schema:PropertyValue
67 N3b2547b3f1fb46e5b43c0c8261681126 rdf:first sg:person.0755076137.76
68 rdf:rest rdf:nil
69 N4998a17796ec4533890f68c5385e0e91 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
70 schema:name Melanocytes
71 rdf:type schema:DefinedTerm
72 N5f93bd12f54742b4b36d842dbe0802a1 schema:name dimensions_id
73 schema:value pub.1008156207
74 rdf:type schema:PropertyValue
75 N9eba758a06f0409dae423fa596fa1011 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Cell Separation
77 rdf:type schema:DefinedTerm
78 Na1f66a75f2124f3bae01caefcb23f2f6 schema:issueNumber 5
79 rdf:type schema:PublicationIssue
80 Na7fa72e628104812885fb56bbc44ca72 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Humans
82 rdf:type schema:DefinedTerm
83 Na80482b380d24f159919d235a5628b2f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Gentamicins
85 rdf:type schema:DefinedTerm
86 Nb400efc170334f12b49630fee3ccbb92 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Cell Division
88 rdf:type schema:DefinedTerm
89 Nb87b0506e6284d43b2aea7b453ee4709 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Kinetics
91 rdf:type schema:DefinedTerm
92 Nc66957ae235843b3ad218ee9fb2e07ed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Fibroblasts
94 rdf:type schema:DefinedTerm
95 Nc7da9e7d13814f81bde45506f9fb8646 schema:volumeNumber 20
96 rdf:type schema:PublicationVolume
97 Nc7ef7ccfbeb446a3bb901ae8184f85d6 schema:name doi
98 schema:value 10.1007/bf02619590
99 rdf:type schema:PropertyValue
100 Ne850655ecb0649648e7634596efbcb2b schema:name Springer Nature - SN SciGraph project
101 rdf:type schema:Organization
102 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
103 schema:name Biological Sciences
104 rdf:type schema:DefinedTerm
105 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
106 schema:name Biochemistry and Cell Biology
107 rdf:type schema:DefinedTerm
108 sg:grant.2465828 http://pending.schema.org/fundedItem sg:pub.10.1007/bf02619590
109 rdf:type schema:MonetaryGrant
110 sg:journal.1312285 schema:issn 0883-8364
111 schema:name In Vitro Cellular & Developmental Biology
112 schema:publisher Springer Nature
113 rdf:type schema:Periodical
114 sg:person.053601122.01 schema:affiliation grid-institutes:grid.47100.32
115 schema:familyName Halaban
116 schema:givenName Ruth
117 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.053601122.01
118 rdf:type schema:Person
119 sg:person.0755076137.76 schema:affiliation grid-institutes:grid.47100.32
120 schema:familyName Alfano
121 schema:givenName Francis D.
122 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755076137.76
123 rdf:type schema:Person
124 grid-institutes:grid.47100.32 schema:alternateName Department of Dermatology, Yale University School of Medicine, 333 Cedar Street, 06510, New Haven, Connecticut
125 schema:name Department of Dermatology, Yale University School of Medicine, 333 Cedar Street, 06510, New Haven, Connecticut
126 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...